| 盐酸法舒地尔在脂肪干细胞向表皮细胞诱导分化中的作用 |
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| 引用本文： | 沙德潜,陈伟.盐酸法舒地尔在脂肪干细胞向表皮细胞诱导分化中的作用[J].新乡医学院学报,2011,28(6):711-713. |
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| 作者姓名： | 沙德潜 陈伟 |
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| 作者单位： | 1. 武警医学院附属医院烧伤整形科,天津,300162
2. 武警医学院附属医院皮肤科,天津,300162 |
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| 摘 要： | 目的探讨盐酸法舒地尔(HA1077)在脂肪干细胞(ADSC)向表皮细胞诱导分化中的作用。方法用酶消化法消化人脂肪组织获取ADSC,以基础培养基即体积分数含10%胎牛血清的DMEM培养基传代,取生长良好达到90%融合的第3代人ADSC分为4组,第1组持续用基础培养基培养、传代;将第2、3、4组弃基础培养基,无菌磷酸盐缓冲溶液冲洗,分别换用培养液2(基础培养基+20μmol.L-1 HA1077)、培养液3(体积分数70%的基础培养基+体积分数30%的成纤维细胞培养基上清液+10 ng.L-1重组人表皮生长因子)、培养液4(20μmol.L-1 HA1077+培养液3),诱导后经免疫细胞化学、流式细胞仪检测CK19的表达。结果免疫细胞化学分析表明ADSC表达CD44、CD49d,而不表达CD106、CD34、CK19;流式细胞仪检测CD44、CD49d的阳性率分别为(98.84±0.02)%、(92.52±0.04)%。流式细胞仪检测各组CK19的表达阳性率分别为(0.23±0.01)%、(0.35±0.02)%、(9.73±0.80)%、(17.65±1.00)%,其中第3、4组CK19的阳性率较对照组明显增高,差别均有统计学意义(P<0.05)。结论 HA1077对ADSC向表皮细胞诱导分化有促进作用。
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| 关 键 词： | 人脂肪干细胞 表皮细胞 盐酸法舒地尔 |
Effect of fasudil hydrochloride inducing adipose derived stem cells to epidermic cells |
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| SHA De-qian,CHEN Wei.Effect of fasudil hydrochloride inducing adipose derived stem cells to epidermic cells[J].Journal of Xinxiang Medical College,2011,28(6):711-713. |
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| Authors: | SHA De-qian CHEN Wei |
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| Institution: | 1.Department of Burn and Plastic Surgery,the Affiliated Hospital of Medical College of Chinese People′s Armed Police Forces,Tianjin 300162,China;2.Department of Dermatology,the Affiliated Hospital of Medical College of Chinese People′s Armed Police Forces,Tianjin 300162,China) |
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| Abstract: | Objective To observe the effect of fasudil hydrochloride(HA1077) inducing adipose derived stem cell(ADSCs) to epidermic cells.Methods The ADSCs were obtained by using enzyme digestion,and then the cells were cultured in vitro.The third generation of ADSCs were chosen from the cells which grew well with a 90% fusion rate,then divided into 4 groups.Group 1 was continuously cultured with culture solution 1:(basic medium);in group 2,3,and 4,solution 1 were washed with sterile phosphate buffered saline and the c... |
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| Keywords: | human adipose derived stem cell epidermic cells fasudil hydrochloride |
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