肝素治疗对重度子痫前期孕妇血清肿瘤坏死因子和白细胞介素6含量的影响

目的观察小剂量肝素对重度子痫前期孕妇血清肿瘤坏死因子(TNF-α)及白细胞介素6(IL-6)水平变化的影响,探讨肝素治疗重度子痫前期的机制。方法重度子痫前期孕妇40例,随机分为治疗组和治疗对照组各20例,治疗组连续7 d每日静脉滴注250 g.L-1硫酸镁60 mL+肝素25 mg;治疗对照组连续7 d每日静脉滴注250 g.L-1硫酸镁60 mL。另选择同期正常晚期妊娠孕妇20例作为正常组。采用酶联免疫双抗体夹心法检测各组孕妇血清TNF-α、IL-6水平变化。结果治疗组与治疗对照组治疗前TNF-α、IL-6比较差别无统计学意义(P>0.05)。与正常组比较,治疗组和治疗对照组治疗前后TNF-α、IL-6含量均明显升高(P<0.01)。治疗组治疗后血清中TNF-α、IL-6含量均低于治疗前,差别有统计学意义(P<0.01);治疗对照组治疗后血清中TNF-α、IL-6含量与治疗前比较差别无统计学意义(P>0.05)。治疗后治疗组血清TNF-α、IL-6较治疗对照组明显降低,差别有统计学意义(P<0.01)。结论炎症反应在重度子痫前期的发病中起一定作用,肝素能通过抗炎机制达到治疗重度子痫前期的作用。

Influences on serum concentrations of tumor necrosis factor-a and interleukin- 6 by heparin treatment for pregnant women with severe preeclampsia

Objective To study the effects of small doses heparin on serum levels of tumor necrosis facter-α（TNF-α） and interleukin-6（IL-6） in patients with severe preeclampsia and evaluate the mechanism of heparin for severe preeclampsia.Methods Forty pregnant women with severe preeclampsia were divided into treatment group and treatment control group,twenty patients in each group.Both groups were intervenous drop infusion MgSO4（250 g·L-1）60 mL for seven days;base on this,treatment group was given heparin 25 mg.Twenty normal late pregnant women were selected as normal control group.The serum concentration of TNF-α and IL-6 in each group were detected by enzyme-linked immunoabsorbant assay（ELISA）.Results The serum concentration of TNF-α,IL-6 before treatment were not significant difference between treatment group and treatment control group（P〈0.05）.The serum concentrations of TNF-α,IL-6 in treatment group and treatment control group were significantly higher than those in normal group（P〈0.01）.The serum levels of TNF-α and IL-6 after treatment were significantly lower than before treatment in treatment group（P〈0.01）.There were not statistic difference of TNF-α and IL-6 levels before and after treatment in treatment control group（P〉0.05）.Compared with treatment control group,the serum concentration of TNF-α,IL-6 in treatment group were decreased after treatment（P〈0.01）.Conclusion Inflammatory reaction play a role in severe preeclampsia,and heparin can treat severe preeclampsia by suppressing inflammation.