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怀山药脂肪酸成分分析
引用本文:王勇,赵若夏,白冰,柳爱莲,刘绣华.怀山药脂肪酸成分分析[J].新乡医学院学报,2008,25(2):112-113.
作者姓名:王勇  赵若夏  白冰  柳爱莲  刘绣华
作者单位:1. 河南大学化学化工学院,河南,开封,475004
2. 河南省济源第一中学,河南,济源,454600
3. 河南大学体育学院,河南,开封,475001
基金项目:河南省高校杰出科研人才基金(编号:2004KYCX018),河南省杰出青年科学基金(编号:0612001100),河南大学校内重点基金(编号:05ZDZR005)
摘    要:目的分离鉴定河南产怀山药(Dioscorea oppositathumb)脂肪酸成分。方法怀山药以石油醚为溶剂进行索氏提取,所得脂肪酸利用硫酸-甲醇法进行甲酯化,利用气相色谱-质谱联用技术对脂肪酸甲酯化产物分离鉴定,应用峰面积归一化法测定各组分相对含量。结果共检出27种脂肪酸,饱和脂肪酸18种,占脂肪酸总量的51%,主要成分为十六酸,其中奇数脂肪酸8种;不饱和脂肪酸9种,占总量的49%,主要为亚油酸、油酸和亚麻酸。结论怀山药中含有较多对人体有益的不饱和脂肪酸和奇数碳脂肪酸,这可能与怀山药的营养保健作用有关。

关 键 词:怀山药  脂肪酸  气相色谱-质谱
文章编号:1004-7239(2008)02-0112-02
收稿时间:2007-08-17
修稿时间:2007年8月17日

Analysis of fatty acids from Dioscorea opposita thunb
WANG Yong, ZHAO Ruo-xia, BAI Bing , et al.Analysis of fatty acids from Dioscorea opposita thunb[J].Journal of Xinxiang Medical College,2008,25(2):112-113.
Authors:WANG Yong  ZHAO Ruo-xia  BAI Bing  
Institution:WANG Yong, ZHAO Ruo-xia, BAI Bing , et al (1. College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China ;2. No. 1 High School of Jiyuan, Jiyuan 454600, China ;3. College of Physical Education, Henan University, Kaifeng 475001, China)
Abstract:Objective To isolate and determine the chemical components of fatty acids from Dioscorea opposita thunb.Methods Dioscorea opposita thunb was extracted with petroleum ether,and then the extract was methylated with sulphuric acid-methanol.The chemical components of fatty acids were analyzed by gas chromatography-mass spectrum(GC/MS),and the relative contents were determined by peak area normalization.Results Twenty-seven fatty acids were identified.Satisfied fatty acid were 18 species(51%),the major components were hexadecanoic,and there were 8 species odd-numbered fatty acid.Unsaturated fatty acidlinoleic acid were 9 species(49%).The major components were linoleic acid,oleic acid and linolenic acid.Conclusion There are various useful fatty acids which are relative to nutritious and health-care function of Dioscorea opposita thunb.
Keywords:Dioscorea opposita thunb  fatty acids  gas chromatography-mass spectrum
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