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肠致病性大肠杆菌毒力因子EspF对肿瘤坏死因子-α、白细胞介素-6和白细胞介素-8的影响
引用本文:王佳贺,任开明,姜洪芳,白雪,吴宝刚,张萌,何平.肠致病性大肠杆菌毒力因子EspF对肿瘤坏死因子-α、白细胞介素-6和白细胞介素-8的影响[J].新乡医学院学报,2011,28(3):265-267.
作者姓名:王佳贺  任开明  姜洪芳  白雪  吴宝刚  张萌  何平
作者单位:1. 中国医科大学附属盛京医院老年病科,辽宁沈阳,110004
2. 中国医科大学附属盛京医院胸外科,辽宁沈阳,110004
基金项目:国家自然科学基金资助项目(编号:30770109); 辽宁省教育厅资助项目(编号:L2010701)
摘    要:目的研究肠致病性大肠杆菌(EPEC)毒力因子EspF转染人肠上皮细胞(HIC)株时,血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和IL-8水平的动态变化及与HIC凋亡的关系。方法以Annexin V FITC/PI双染流式细胞仪检测HIC的凋亡率,并用酶联免疫吸附测定法检测转染0、6、12、24、48 h细胞培养上清液中TNF-α、IL-6和IL-8浓度的变化。结果 EspF转染HIC后TNF-α、IL-6和IL-8活性均显著增加,HIC凋亡率在转染后逐渐增高,HIC凋亡率与TNF-α、IL-6和IL-8的水平呈正相关。结论 EspF可激活多种炎症介质和诱导HIC凋亡,EspF转染HIC后TNF-α、IL-6和IL-8水平的升高在EPEC致病过程中起重要作用。

关 键 词:肠致病性大肠杆菌  肿瘤坏死因子-α  白细胞介素-6  白细胞介素-8  人肠上皮细胞  凋亡

Effect of virulence factor EspF from enteropathic E.coli on tumor necrosis factor-α,interleukin-6 and interleukin-8
WANG Jia-he,REN Kai-ming,JIANG Hong-fang,BAI Xue,WU Bao-gang,ZHANG Meng,HE Ping.Effect of virulence factor EspF from enteropathic E.coli on tumor necrosis factor-α,interleukin-6 and interleukin-8[J].Journal of Xinxiang Medical College,2011,28(3):265-267.
Authors:WANG Jia-he  REN Kai-ming  JIANG Hong-fang  BAI Xue  WU Bao-gang  ZHANG Meng  HE Ping
Institution:WANG Jia-he1,REN Kai-ming2,JIANG Hong-fang1,BAI Xue1,WU Bao-gang1,ZHANG Meng1,HE Ping1(1.Department of Geriatrics,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China,2.Department of Thoracic Surgery,China)
Abstract:Objective To investigate the relationship between the apoptosis of human intestinal epithelial cells(HIC) and the dynamic changes of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and interleukin-8(IL-8) level when enteropathic E.coli(EPEC) virulence factor carried HIC strain.Methods The apoptosis rate of HIC was detected by Annexin V FITC /PI double dye flow cytometric analysis.The content changes of TNF-α,IL-6 and IL-8 in the supernatant of the cell culture were assessed by enzyme-linked immunosorbent assay(ELISA) at 0,6,12,24 and 48 hours after transfection.Results The activities of TNF-α,IL-6 and IL-8 were increased significantly after EspF transfected HIC,and the apoptosis rate of HIC was increased gradually after transfection.The levels of TNF-α,IL-6 and IL-8 had positive correlation with the apoptosis rate of HIC.Conclusion EspF can activate many inflammatory medium,and induce HIC apoptosis.The level of TNF-α,IL-6 and IL-8 increased after EspF transfected HIC cells which played an important role in the immunopathogenesis of EPEC infection.
Keywords:enteropathic E  coli  tumor necrosis factor-α  interleukin-6  interleukin-8  human intestinal epithelial cells  apoptosis
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