不稳定性心绞痛患者基质金属蛋白酶-1与CD40配体的关系研究

目的检测不稳定性心绞痛(UA)患者外周血基质金属蛋白酶-1(MMP-1)、细胞分化抗原40配体(CD40L)、白介素-1β(IL-1β)、C-反应蛋白(CRP)水平的变化,分析MMP-1水平与CD40L及IL-1β的相关性,探讨CD40L介导MMP-1的表达与分泌在UA发病机制中所起的作用。方法选择UA患者(UA组)32例,稳定性心绞痛(SA)患者(SA组)28例及健康对照组20例,采用酶联免疫吸附法(ELISA)分别测定各组外周血可溶性CD40L、MMP-1及IL-1β的水平,采用免疫比浊法测定各组CRP的水平,分析UA组MMP-1与CD40L及IL-1β之间的相关性,分析MMP-1水平与CRP的相关性。结果UA组MMP-1、CD40L和IL-1β水平显著高于SA组和对照组(P<0.01);UA组MMP-1与CD40L水平之间呈显著正相关(r=0.486,P<0.01),MMP-1与IL-1β水平之间呈相对较弱正相关(r=0.369,P<0.05);UA组MMP-1水平与CRP之间呈正的直线相关(r=0.489,P<0.01)。结论冠状动脉粥样硬化斑块的破裂可能与CD40L介导的MMP-1表达与分泌有关。

Relationship between level of matrix metalloproteinase-1 and soluble CD40 ligand in patients with unstable angina

Objective To analyze the correlation of metalloproteinase-1(MMP-1)and CD40 ligand(CD40L)or MMP-1 and IL-1β,so as to explore the role of expression and secretion of MMP-1 regulated by CD40L on pathophysiologicial mechanism of unstable angina.Methods The serum levels of soluble CD40L,MMP-1 and IL-1β were measured by enzyme-linked immunosorbent assay in 36 patients with unstable angina,24 patients with stable angina and 20 controls.And also the levels of Creactive protein(CRP) were measured by immune-turbidimetry method,and the correlations of MMP-1 and CD40L or IL-1β were analyzed,and to analyze the correlation of MMP-1 and CRP in patients with unstable angina.Results Serum levels of MMP-1,CD40L and IL-1β were significantly higher in patients with unstable angina than those with stable angina(P<0.01) and controls(P<0.01).MMP-1 level was stronger positively correlated with CD40L in unstable angina(r=0.486,P<0.01),MMP-1 was positively weaker correlated with IL-1β(r=0.369,P<0.05).Level MMP-1 were positively correlated with CRP in patients with unstable angina(r=0.489,P<0.01).Conclusion It is possible reason for the expression and secretion of MMP-1 regulated by CD40 L in the rupture of coronary artery plaque.