食管鳞状细胞癌中基质金属蛋白酶-2和Survivin的表达及其意义

目的研究基质金属蛋白酶-2(MMP-2)和Survivin在食管鳞状细胞癌(ESCC)中的表达及其与临床病理特征之间的关系。方法采用免疫组织化学Elivision非生物素检测方法,检测MMP-2和Survivin蛋白在58例ESCC和30例癌旁正常食管上皮中的表达。结果MMP-2和Survivin蛋白在ESCC中阳性表达率均高于食管正常上皮(P<0.01);MMP-2蛋白表达与浸润深度、淋巴结转移和病理分级有显著相关性(P<0.01),随着肿瘤的浸润深度、临床病理分级的提高与区域淋巴结转移而上调;Survivin蛋白表达与浸润深度和淋巴结转移有显著相关性(P<0.01),而与病理分级无显著相关性(P>0.05);MMP-2表达与Survivin表达呈正相关(P<0.05)。结论MMP-2和Survivin的表达在ESCC的发生、发展中起重要作用,对ESCC的早期诊断、判断预后有重要参考价值。

Expression of matrix metalloproteinase-2 and Survivin in esophageal squamous cell carcinoma

ObjectiveTo investigate the expression of matrix metalloproteinase-2(MMP-2) and survivin proteins in esophageal squamous cell carcinoma(ESCC),and to analyze the relationship between the expressions and clinicopathologic factors.MethodsThe expression of MMP-2 and survivin protein was detected with immunohistochemical ElivisionTM plus non-biotin technique in 58 cases of ESCCs and 30 cases of normal tissues around tumour.ResultsThe positive expression rates of MMP-2 protein and survivin protein in ESCC were significantly higher than those in normal epithelium(P<0.01),respectively.MMP-2 protein expression was markedly correlated with tumor infiltration depth,lymph node metastasis and tumor differentiation degree(P<0.01).Survivin protein expression was also positively correlated with tumor infiltration depth and lymph node metastasis(P<0.01),but it was incorrelated with tumor differentiation degree(P>0.05).MMP-2 protein expression was positively correlated with survivin(P<0.01).ConclusionMMP-2 and Survivin may play important roles in the carcinogenesis and development of ESCC.Combinative detecting the expression of MMP-2 and Survivin may provide more accurate information in early diagnosis and predicting the prognosis of ESCC.