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煤焦沥青烟提取物致大鼠肺泡巨噬细胞DNA损伤作用
引用本文:李志春,吴逸明,徐玉宝,陈琛.煤焦沥青烟提取物致大鼠肺泡巨噬细胞DNA损伤作用[J].新乡医学院学报,2001,18(4):248-250.
作者姓名:李志春  吴逸明  徐玉宝  陈琛
作者单位:1. 新乡医学院预防医学教研室
2. 河南医科大学公共卫生学院劳动卫生学教研室
摘    要:目的 研究煤焦沥青烟提取物致大鼠肺泡巨噬细胞 (AM )DNA损伤作用。方法 将大鼠肺泡巨噬细胞分 4组与不同浓度的煤焦沥青烟提取物共育 1h后 ,采用单细胞凝胶电泳技术检测大鼠肺泡巨噬细胞DNA断裂情况。结果 单细胞凝胶电泳后 ,对照组肺泡巨噬细胞无明显细胞拖尾现象 ,而经不同浓度的煤焦沥青烟提取物处理后 ,AM细胞有明显的拖尾现象 ,且随煤焦沥青烟提取物浓度增加 ,AM细胞彗星率上升 ,各组间差异有高度显著性 (P <0 .0 0 1) ,而且损伤的严重程度与作用物浓度呈相关关系 (Spearman相关系数为 0 .6 73,P <0 .0 0 1)。结论 煤焦沥青烟提取物作用于大鼠肺泡巨噬细胞可引起DNA链的断裂 ,单细胞凝胶电泳适于检测肺泡巨噬细胞的DNA链断裂

关 键 词:单细胞凝胶电泳  煤焦沥青  DNA损伤  肺泡巨噬细胞
文章编号:1004-7239(2001)04-0248-03
修稿时间:2001年6月18日

DNA damage of alveolar macrophage induced by extracts of coal tar pitch fume in rat
LI Zhi-chun,WU Yi-ming,XU Yu-bao,CHEN Chen.DNA damage of alveolar macrophage induced by extracts of coal tar pitch fume in rat[J].Journal of Xinxiang Medical College,2001,18(4):248-250.
Authors:LI Zhi-chun  WU Yi-ming  XU Yu-bao  CHEN Chen
Institution:LI Zhi-chun1,WU Yi-ming2,XU Yu-bao2,CHEN Chen2
Abstract:Objective To study the DNA damage of alveolar macrophage induced by extracts of coal tar pitch fume in rat. Methods The alveolar macrophages (AM) were divided into 4 groups. These cells were exposured to 2.5?μg·ml -1 , 5.0?μg·ml -1 and 10.0?μg·ml -1 extracts of coal tar pitch fume and a control group. After incubating 1 hours, the single cell gel electrophoresis(SCGE) were used to detect the DNA strand breaks of AM. Results After undergoing electrophoresis, the "comet" cells were seldom in control group, but the "comet" cells increased significantly in experimental groups. There were significant differences in all groups(P<0.001). Conclusions Coal tar pitch fume extracts can induce DNA single strand breaks in alveolar macrophage of rat, and SCGE is suitable for the detection of DNA strand breaks in alveolar macrophage of rat.
Keywords:single cell gel electrophoresis  coal tar pitch  DNA damage  alveolar macrophage
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