不同抑制剂对亚硒酸钠作用下宁夏枸杞抗氧化系统的影响

目的研究不同抑制剂对亚硒酸钠(Na2SeO3)作用下宁夏枸杞抗氧化系统的影响。方法将宁夏枸杞随机分为对照组8株、Na2SeO3组8株、放线菌素D(AMD)组16株(3μg.L-1AMD组和5μg.L-1AMD组各8株)及环己亚胺(CHM)组16株(0.75μg.L-1CHM组和1.5μg.L-1CHM组各8株)。Na2SeO3组、3μg.L-1AMD组、5μg.L-1AMD组、0.75μg.L-1CHM组和1.5μg.L-1CHM组分别于发芽期和开花期每千克土壤施用1.0 mg.L-1的Na2SeO3各1 L;同时AMD 3μg.L-1组和AMD 5μg.L-1组每千克土壤施用3μg.L-1和5μg.L-1的AMD各1 L,0.75μg.L-1CHM组和1.5μg.L-1CHM组每千克土壤施用0.75μg.L-1和1.5μg.L-1的CHM各1 L;对照组不施用AMD、CHM和Na2SeO3。于同日采摘各组的枸杞果实,并及时进行谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性以及谷胱甘肽(GSH)、抗坏血酸(Asc)含量的测定。结果与对照组比较,Na2SeO3组和3μg.L-1AMD组SOD、POD、GSH-Px活性以及GSH、Asc含量明显提高(P<0.05或P<0.01);5μg.L-1AMD组POD活性、GSH-Px以及GSH含量明显提高(P<0.05);0.75μg.L-1CHM组SOD活性、GSH-Px及GSH、Asc含量明显提高(P<0.05)。与Na2SeO3组比较,3μg.L-1AMD组GSH-Px活性和5μg.L-1AMD组SOD、GSH-Px活性和GSH、Asc含量显著降低(P<0.05);0.75μg.L-1CHM组GSH-Px活性、GSH含量以及1.5μg.L-1CHM组SOD、POD活性、GSH-Px和GSH、Asc含量明显降低(P<0.05)。与3μg.L-1AMD组比较,5μg.L-1AMD组GSH-Px活性、GSH含量降低(P<0.05)。与0.75μg.L-1CHM组比较,1.5μg.L-1CHM组POD活性、GSH-Px及GSH、Asc含量明显降低(P<0.05)。结论Na2SeO3能够提高宁夏枸杞的抗氧化性,而AMD和CHM都能够降低Na2SeO3作用下宁夏枸杞的抗氧化功效。

Effects of different inhibitor on the antioxidant system of Lycium barbarums on the function of Na2SeO3

Objective To investigate the effects of different inhibitors on the antioxidant system of Lycium barbarums on the function of Na2SeO3.Methods Lycium barbarums were randomly divided into four groups：control group was 8 Lycium barbarums）,Na2SeO3 group was 8 Lycium barbarums,actinomycin D（AMD） group was 16 Lycium barbarums（3 μg·L^-1 AMD group and 5 μg·L^-1 AMD group were respectively 8 Lycium barbarums）,cycloheximide（CHM） group was 16 Lycium barbarums（0.75 μg·L^-1 CHM group and 1.5 μg·L^-1 CHM group were respectively 8 Lycium barbarums）.Na2SeO3 1 L（1.0 mg·L^-1）was respectively used in emergence time and flowering period in Na2SeO3 group,3 μg·L^-1 AMD group,5 μg·L^-1AMD group,CHM0.75 μg·L^-1 group and 1.5 μg·L^-1 CHM group.While 3 μg·L^-1 and 5 μg·L^-1 AMD were used in 3 μg·L^-1 AMD group and 5 μg·L^-1 AMD group for 1 L respectively,0.75 μg·L^-1 and 1.5 μg·L^-1 CHM were used in 0.75 μg·L^-1 CHM group and 1.5 μg·L^-1 CHM group for 1L respectively.Control group was no AMD,CHM and Na2SeO3.The activities of glutathione peroxidase（GSH-Px）,superoxide dismutase（SOD）,peroxidase（POD） and the contents of glutathione（GSH）,ascorbate（Asc） were determined when the fruit of each group was picked at the same time.Results Compared with control group,activities of SOD,POD,GSH-Px and contents of GSH and Asc in Na2SeO3 group and 3 μg·L^-1 AMD group increased obviously（P〈0.05）.Activities of POD and GSH-Px and contents of GSH in 5 μg·L^-1 AMD group were increased（P〈0.05）.Activities of SOD,GSH-Px and contents of GSH,Asc in 0.75 μg·L^-1 CHM group were increased（P〈0.05）.Compared with Na2SeO3 group,activity of GSH-Px in 3 μg·L^-1 AMD group,activities of SOD,GSH-Px and contents of GSH,Asc in 5 μg·L^-1AMD group were decreased（P〈0.05）.Activity of GSH-Px and content of GSH in 0.75 μg·L^-1CHM group,activities of SOD、POD and GSH-Px,and contents of GSH,Asc in 1.5 μg·L^-1 CHM group was decreased（P〈0.05）.Compared with 3 μg·L^-1A