高脂诱导非酒精性脂肪性肝病小鼠肝脏Chemerin及其受体的表达

目的研究高脂饮食诱导非酒精性脂肪性肝病(NAFLD)小鼠肝脏Chemerin及Chemerin受体(CMKLR1)的表达情况。方法22只C57BL/6小鼠随机分为对照组(n=10)和高脂组(n=12),对照组给予基础饲料喂养,高脂组给予高脂饲料喂养,连续喂养18周后进行腹腔注射葡萄糖耐量试验(IPGTT)和胰岛素耐量试验(ITT),检测血清游离脂肪酸(FFA)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、谷丙转氨酶(ALT);ITT结束后第3天处死小鼠,测肝脏TG含量,并做HE染色观察肝脏形态变化;采用实时荧光定量聚合酶链反应(RT-PCR)检测细胞因子Chemerin、CMKLR1及肿瘤坏死因子-α(TNF-α)mRNA水平的表达。结果高脂组与对照组相比,胰岛素的敏感性下降(P<0.05),肝脏HE染色存在明显的炎症及肝细胞脂肪变,肝脏的TG含量升高(P<0.05),血脂紊乱及肝功能损伤,肝脏Chemerin的mRNA水平下降(P<0.05),CMKLR1及TNF-α的mRNA水平表达上升(P<0.05)。结论经高脂喂养18周后小鼠NAFLD造模成功并伴有胰岛素抵抗,在这种模型中肝脏Chemerin及CMKLR1的表达改变可能与NAFLD的发病有关。

Expression of Chemerin and it's receptor in liver of mouse with nonalcoholic fatty liver disease induced by high fat diet

Objective To investigate the expression of Chemerin and Chemerin receptor （Chemerin-like-receptorl, CMKLR1 ） in liver of mouse with nonalcoholic fatty liver disease （NAFLD） induced by high fat diet. Methods 22 C57BL/6 mice were randomly divided into control group（ n = 10 ）which were given common feed and high fat diet group （n = 12 ）which were given high fat diet. Eighteen weeks later, intraperitoneal glucose tolerance test （IPGTr） and insulin tolerance test（ITT） were performed for estimating insulin sensitivity, and all the mice were executed three days after ITT. The levels of serum free fatty acid （ FFA）, total cholesterol （ TC ）, triglyceride （ TG）, low density lipoprotein （ LDL）, high density lipoprotein （ HDL）, alanine aminotransferase（ALT） and the content of liver TG were detected and HE dying was done to observe the morphologic change of liver. The mRNA expression levels of Chemerin, CMKLR1 and tumor necrosis faetor-α（TNF-α） in liver were measured by real-time polymerase chain reaction（ RT-time PCR）. Results Compared with control group, the insulin sensitivity, expression levels of Chemerin mRNA in liver was lower（ P 〈 0.05 ） and TG contents in liver, CMKLR1 and TNF-α mRNA level were higher in high fat diet group （ P 〈 0.05 ）. Conclusion After 18 weeks, the model of nonalcoholic fatty liver disease was constructed successfully with insulin resistance. The change of Chemerin and Chemerin receptor expression levels in mouse liver may be related to the development of nonalcoholic fatty liver disease induced by high fat diet.