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脓毒症大鼠Fas-L、Caspase-3和NF-κB的表达与肝细胞凋亡的关系
引用本文:高建芝,张建龙,王红梅,马琪,腾清蕾.脓毒症大鼠Fas-L、Caspase-3和NF-κB的表达与肝细胞凋亡的关系[J].新乡医学院学报,2004,21(2):96-99.
作者姓名:高建芝  张建龙  王红梅  马琪  腾清蕾
作者单位:新疆医科大学病理生理教研室,新疆,乌鲁木齐,830054
摘    要:目的 探讨脓毒症时Fas L、Caspase 3、NF κB在肝细胞凋亡信号转导中的作用。方法 Wistar大鼠 3 0只随机分为观察组和对照组 ,观察组 2 4只采用盲肠结扎穿孔术 (CLP)致脓毒症模型 ,以术后 2h、3h、4h、5h时限分为 4组 ,对照组 6只 ,不行CLP术。各组均测定肝脏组织Fas L、Caspase 3、NF κB蛋白表达及肝细胞凋亡的情况 ,同时测定肝脏功能的变化。结果 CLP术后肝组织Fas L、Caspase 3、NF κB蛋白表达升高与对照组相比有显著差异 ;3h组的Fas L、Caspase 3的含量达高峰 ,4h组的NF κB的含量达高峰 ;血浆中丙氨酸转氨酶 (ALT)、乳酸脱氢酶 (LDH)呈异常改变 ,CLP术后血浆中ALT、LDH增加 ,与对照组相比有显著差异 (P <0 .0 1) ,ALT呈进行性增加 ,LDH以 4h组最显著。肝组织细胞凋亡指数呈进行性增加。结论 由CLP所致的脓毒症过程中 ,肝组织Fas L、Caspase 3蛋白表达升高 ,激活促细胞凋亡的程序 ;NF κB蛋白表达亦升高 ,NF κB活性增强 ,抑制细胞凋亡

关 键 词:脓毒症  Fas-L  Caspase-3  NF-κB  肝细胞  细胞凋亡
文章编号:1004-7239(2004)02-0096-04
修稿时间:2004年1月5日

Relationship of expression of Fas-L,Caspase-3 and NF-κB and hepatocyte apoptosis in septic rat
GAO Jian-zhi,ZHANG Jian-long,WANG Hong-mei,et al.Relationship of expression of Fas-L,Caspase-3 and NF-κB and hepatocyte apoptosis in septic rat[J].Journal of Xinxiang Medical College,2004,21(2):96-99.
Authors:GAO Jian-zhi  ZHANG Jian-long  WANG Hong-mei  
Abstract:Objective To investigate the role of Fas-L,Caspase- 3 and NF-κB in signal transduction in heptocyte apoptosis.Methods Thirty Wistar rats were randomly divided into the normal control amd the exp erimental group.Twenty-four of them were subjected to septic model induced by c ecal ligation and puncture (CLP),and they were respectively sacrificed at 2 h,3 h,4 h,5 h after CLP.The levels of expression of Fas-L,Caspase-3 and NF-κB protein in liver tissues and hepatocyte apoptosis were measured at various intervals in liver tissues. Alanine aminotransferase(ALT) and lactate dehydrogenase(LDH) wer e also detected at the same time.Results Expression of Fas-L, Caspase-3 and NF-κB protein in liver tissues significantly increased compare d with the control group.Content of Fas-L,Caspase-3 of the 3-h group approach ed the peak.Content of NF-κB of the 4-h group approached the peak.Meanwhile,s erum ALT and LDH were markedly increased compared with the control group(P< 0.01),serum ALT showed a progressive increment,and increment of activity of seru m LDH in the 4-h group was prominently,Index of hepatocyte apoptosis showed a progressive increment.Conclusion The expression of Fas-L and Caspase-3 protein increased with activated apoptosis during sepsis induced by CLP;the activity of NF-κB protein was increased as well, and it might suppres se hepatocyte apoptosis.
Keywords:sepsis  Fas-L  Caspase-3  NF-κB  hepatocyte  apoptos is
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