| 蓝氏贾第鞭毛虫承德株分子生物学研究 |
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| 引用本文： | 陈小宁,卢思奇.蓝氏贾第鞭毛虫承德株分子生物学研究[J].承德医学院学报,2000,17(4):93-96. |
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| 作者姓名： | 陈小宁 卢思奇 |
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| 作者单位： | [1]承德医学院人本寄生虫学教研室,河北承德 [2]首都医科大学人体寄生虫学教研室,河北承德 |
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| 摘 要： | 目的：探讨蓝氏贾第鞭毛虫大、小型包囊存在的原因。方法：用分子生物学技术对３株贾第虫（ＣＨ２、ＣＨ３、ＣＨ４）进行ｔｉｍ基因扩增和序列测定。结果：经与ＧｅｎＢａｎｋ登记虫株比较和用ＤＮＡｓｔａｒ软件处理的结果表明，此３株贾第虫的基因型属２型（ＪＨ型）。结论：大、小型包裹在形态学上经过统计学处理显示有显著差异，但与基因型无关。
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| 关 键 词： | 蓝氏贾第鞭毛虫病 tim基因 分子生物学 |
INVESTIGATION OF CHENGDE ISOLATES OF GIARDIA LAMBLIA ON THEIR MOLECULARBIOLOGY |
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| GHEN Xiao-ning,et al.INVESTIGATION OF CHENGDE ISOLATES OF GIARDIA LAMBLIA ON THEIR MOLECULARBIOLOGY[J].Journal of Chengde Medical College,2000,17(4):93-96. |
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| Authors: | GHEN Xiao-ning et al |
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| Abstract: | Objective:To study mechanism of the big and small Giardia lamblia cysts.Methods:Genomic DNA extracted from three isolates were amplified for tim gene sequencing and analysis using molecularbiological technique.Results:Tim gene sequence were compared with those from the isolates registered in GenBank and analysed using DNAstar softwire.It was shown shat three chengde isolates of G.lamblia were fit into group 2(JH).Conclusion:Two Giardia lamblia cysts(the big and the small) differ greatly in the sizes,but there was no relationship between the sizes of Giardia cysts and their genetypes. |
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| Keywords: | Giardia lamblia Tim gene Genetype Gene amplification Sequence analysis |
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