红细胞γ射线辐照时机及辐照后保存的研究

目的研究不同辐照时机对红细胞活性和功能的影响,确定最佳辐照时机和辐照后的保存时间。方法将10份采集的400ml全血制备成红细胞悬液后分成6个组,分别在采血后保存0,7,14,21,28d进行25Gyγ射线辐照(对照组除外);在辐照后0,7,14,21,28,35d分别取样检测2,3-DPG、ATP、游离血红蛋白、K+和红细胞渗透脆性的变化。结果各辐照组2,3-DPG含量与对照组比较差异均无统计学意义(P>0.05);第二十一天辐照组和第二十八天辐照组在辐照后的ATP含量均低于对照组(P<0.05);第七天、第十四天、第二十一天和第二十八天辐照组红细胞最小抵抗力分别自辐照后28,14,7,7d起低于对照组(P<0.05),第十四天、第二十一天和第二十八天辐照组红细胞最大抵抗力分别自辐照后21,7,7d起低于对照组(P<0.05);第二十一天辐照组和第二十八天辐照组游离血红蛋白含量明显高于对照组(P<0.05);各辐照组在辐照后7d起的K+含量迅速上升,且在辐照后所有保存时段内的K+含量均显著高于对照组(P<0.001)。结论红细胞辐照时机直接影响辐照后保存的血液质量及保存时间,建议将红细胞辐照最佳时机定为血液采集后14d内,辐照后的血液可继续保存14～21d。

STUDY ON THE IRRADIATION TIME AND STORAGE PERIOD OF IRRADIATED RED BLOOD CELLS COMPONENTS TREATED WITH GAMMA IRRADIATION

Objective To measure the effects of gamma irradiation on red blood cells(RBC)components and assess the appropriate irradiation time and storage period of irradiated RBC components.Methods RBC components was divided into 6 groups and treated with 25Gy gamma irradiation on day 0,7,14,21 and 28 after collection(except control).On day 0,7,14,21,28 and 35 after irradiation,samples were collected and measured for 2,3-DPG,ATP,K+,plasma free hemoglobin and RBC brittleness.Results 2,3-DPG were not affected in irradiation time during the storage period(P>0.05).ATP levels in day 21 and day 28 irradiation group were lower than the control(P<0.05).The lowest resistance ability of RBC on day 7,14,21 and 28 irradiation groups were lower than the control since day 28,14,7 and 7 after irradiation respectively(P<0.05);The largest resistance ability of RBC on day 14,21 and 28 irradiation groups were lower than the control since day 28,7 and 7 after irradiation respectively(P<0.05).The free hemoglobin levels on day 21 and 28 irradiation groups were higher than the control(P<0.05).Free K+ was increased more rapidly in all irradiation groups than the control since day 7 after irradiation(P<0.001).Conclusion Storage period of irradiated RBC components are affected by irradiation time after collection.It is recommended that RBC components treated with 25Gy gamma irradiation in 14 days after collection be stored from 14 days to 21 days continuously.