邻苯二甲酸二乙己酯及双酚A联合染毒对大鼠肝脏的影响

目的研究邻苯二甲酸二乙基己酯（Di-2-ethylhexyl phthalate,DEHP）和双酚A（Bisphenol A,BPA）联合暴露对大鼠肝脏的影响。方法将Wistar大鼠随机分4组,分别灌胃1 500mg/kg DEHP、200mg/kg BPA及750mg/kg DEHP＋100mg/kg BPA染毒6周。染毒终点测定血清中碱性磷酸酶（ALP）、胆固醇（CHO）、甘油三酯（TG）及镁离子（Mg2＋）含量;检测肝匀浆中谷胱甘肽（GSH）、丙二醛（MDA）含量。结果与对照组比较,DEHP明显升高血清ALP、CHO及Mg2＋含量,而BPA明显增加肝中MDA含量（均P〈0.05）。与对照组比较,DEHP＋BPA联合染毒除明显增加肝脏脏器系数外,对大鼠体重、肝MDA、血脂及血清Mg2＋和ALP含量均无明显影响。与DEHP＋BPA联合染毒组比较,BPA显著升高肝脏MDA含量;DEHP增加血清Mg2＋含量差异均有统计学意义（均P〈0.05）。结论 DEHP和BPA单独染毒均可产生肝损伤,其联合暴露产生交互作用。

COMBINED EFFECT OF PHTHALATE AND BPA ON LIVER IN RATS

Objective To study the combined effect of diethylhexyl phthalate（DEHP）and bisphenol A（BPA）on liver in rats.Methods All the Wistar rats were randomly divided into 4 groups and respectively were gavaged for 6-week with1 500mg/kg DEHP,200mg/kg BPA and 750mg/kg DEHP plus 100mg/kg BPA.The concentrations of alkaline phosphatase（ALP）,cholesterol（CHO）,triglycerides（TG）and magnesium（Mg2＋）in serum were determined.The contents of glutathione（GSH）and malondialdehyde（MDA）in liver homogenate were detected.Results Compared with the control group, DEHP significantly increased the content of ALP,CHO and Mg2＋in serum,and the concentration of hepatic MDA was significantly higher than the BPA group（all P〈0.05）.DEHP＋BPA had no obviously effect on rat weight,MDA concentration in liver homogenate,lipids,Mg2＋and ALP in serum other than the coefficient of liver.Compared with DEHP＋BPA combined exposure group,there was significant increase in MDA concentration in liver homogenate of BPA group and Mg2＋of DEHP group（P〈0.05）.Conclusion Both DEHP and BPA can induce liver damage,and the combined exposure has interaction effect.