旱莲草对老龄小鼠脾淋巴细胞功能的影响

目的探讨旱莲草（HEP）对老龄小鼠脾淋巴细胞功能的影响。方法观察老龄小鼠服旱莲草水煎剂后，脾淋巴细胞IL2、MAF的分泌和DNA合成的变化；早莲草体外对脾淋巴细胞DNA合成的影响。结果1．使老龄小鼠脾淋巴细胞分泌IL－2逐渐减少（P＜0．01），并呈剂量依赖关系（r＝－0．8988）；2．体内外实验表明高浓度旱莲草水煎剂抑制脾淋巴细胞在ConA激活后的DNA合成，但无ConA时DNA合成不受影响；3．老龄小鼠脾淋巴细胞分泌MAF能力明显低于中龄小鼠（PMO．05），灌服旱莲草24g／kg×7d使老龄小鼠脾淋巴细胞分泌MAF能力明显增强（P＜0．05）。结论HEP能明显促进老龄小鼠分泌MAF，说明HEP能提高老龄小鼠的非特异免疫功能。

BIOLOGICAL ACTIVITY OF HEP ON THE FUNCTION OF SP LEN OLYM PHOCYTES IN THE AGED MICE

In order to study the biological activity of elipta extracts on the function of immunology and splenolyphocytes in aged mice. Method The secretions of splenolyphocyte IL-2 and MAF as well as the changes of DNA synthesis were closely examined in the aged mice after administration of HEP decoctum with different concentration. Result A gradual decrease was shown in ILa 2 of splenolyphocytes in the aged mice together with a dosedependent relationship (P<0. 001;r=-0. 8988). Boiled HEP decoctum with high concentration suppressed DNAsynthesis with Con A and the suppression disappeared in the absence of Con A. Aged mice were less able to secrete MAF than the middle-aged mice (P<0. 05), but the secreting ability was obviously increased (P<0. 05) after the administration of HEP. Conclusion HEP can obviously enhance the ability of the aged mice to secrete MAF (P<0. 01),which indicates that HEP is effective in the increasing of aged mice's nonspecific immunologic function.