| 富血小板纤维蛋白超微结构观察及意义 |
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| 引用本文： | 陈铁楼,江一峰,张新海,陈骏,黄传梅,王晓熙.富血小板纤维蛋白超微结构观察及意义[J].同济大学学报(医学版),2018,39(4):46-50. |
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| 作者姓名： | 陈铁楼 江一峰 张新海 陈骏 黄传梅 王晓熙 |
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| 作者单位： | 海军军医大学附属长海医院全军口腔中心牙周科,上海200081；海军军医大学附属长海医院精准医学中心,上海200081,海军军医大学附属长海医院全军口腔中心牙周科,上海200081,海军军医大学附属长海医院全军口腔中心牙周科,上海200081；海军军医大学附属长海医院精准医学中心,上海200081,海军军医大学附属长海医院全军口腔中心牙周科,上海200081,海军军医大学附属长海医院全军口腔中心牙周科,上海200081,海军军医大学附属长海医院病理科,上海200081 |
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| 基金项目： | 上海市卫计委项目（20134418）；中国人民解放军总后勤部项目（CHJ13J035） |
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| 摘 要： | 目的 探讨富血小板纤维蛋白（platelet rich fibrin, PRF）形态学超微结构及意义。方法 选择10例健康志愿者，抽取静脉血制备PRF，对PRF分别行光学显微镜、扫描电子显微镜（scanning electron microscope, SEM）和透射电子显微镜（transmission electron microscope, TEM）观察。结果 PRF为淡黄色纤维蛋白凝胶，H-E染色见胶原纤维为染色均一的淡红色网状结构，在近细胞端纤维排列较致密，含大量血小板。吉姆萨染色见淡蓝色的胶原纤维为网状结构，近细胞端见染为蓝色的白细胞。SEM发现胶原纤维排列成疏松多孔网状支架结构，含大量静止或伸出伪足的血小板及白细胞。TEM发现血小板处于静止或激活状态，α-颗粒释放于细胞和纤维之间，在细胞之间见高密度区为血小板释放的颗粒。结论 PRF胶原纤维的网状立体结构和富含血小板激活释放生长因子，对生物学作用发挥有重要意义。
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| 关 键 词： | 富血小板纤维蛋白 形态学 超微结构 扫描电镜 透射电镜 |
| 收稿时间： | 2017/12/18 0:00:00 |
Morphological ultrastructure of platelet rich fibrin and its implication |
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| CHEN Tie-lou,JIANG Yi-feng,ZHANG Xin-hai,CHEN Jun,HUANG Chuan-mei and WANG Xiao-xi.Morphological ultrastructure of platelet rich fibrin and its implication[J].Journal of Tongji University(Medical Science),2018,39(4):46-50. |
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| Authors: | CHEN Tie-lou JIANG Yi-feng ZHANG Xin-hai CHEN Jun HUANG Chuan-mei and WANG Xiao-xi |
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| Institution: | Dept. of Periodontal, Oral Research Center of CPLA, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China; Precision Medical Center, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China,Dept. of Periodontal, Oral Research Center of CPLA, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China,Dept. of Periodontal, Oral Research Center of CPLA, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China; Precision Medical Center, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China,Dept. of Periodontal, Oral Research Center of CPLA, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China,Dept. of Periodontal, Oral Research Center of CPLA, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China and Dept. of Pathology, Changhai Hospital of PLA Naval Medical University, Shanghai 200081, China |
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| Abstract: | |
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| Keywords: | platelet rich fibrin morphology ultrastructure scanning electron microscope transmission electron microscope |
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