核素介导生长抑素类似物对非小细胞肺癌细胞的体外杀伤作用

目的观察核素介导生长抑素类似物对非小细胞肺癌(non-small celllung cancer,NSCLC)细胞的体外杀伤作用。方法 MTT法测定核素介导生长抑素类似物对人肺癌细胞A549和SPC-A1增殖的影响,流式细胞术检测细胞凋亡,Transwell侵袭实验验证核素介导生长抑素类似物对肿瘤侵袭的影响。结果 2~6 d内,核素介导生长抑素类似物对A549和SPC-A1细胞的抑制率随时间延长明显增加。经核素介导生长抑素类似物处理48 h后,A549和SPC-A1细胞凋亡率分别为23.1%和22.6%。与对照组相比,核素介导生长抑素类似物处理过的A549和SPC-A1细胞侵入胶原的数量减少了约1/3。结论核素介导生长抑素类似物具有诱导NSCLC细胞凋亡的作用,这可能为NSCLC的放射治疗提供了新的思路。

Nuclide-mediated somatostatin analogues induces apoptosis of non-small cell lung cancer cells in vitro

Objective To investigate the effect of nuclide-mediated somatostatin analogues on non-small cell lung cancer(NSCLC) cells in vitro. MethodsCultured NSCLC A549 and SPC-A1 cells were treated with nuclide-mediated somatostatin analogues. Cell proliferation was determined by MTT method, cell apoptosis was detected by flow cytometry, and cell invasion ability was measured by Transwell migration assay. ResultsNuclide-mediated somatostatin analogues inhibited proliferation of A549 and SPC-A1 cells in a time-dependent manner within 2-6 d. The apoptosis rates of A549 and SPC-A1 cells treated by nuclide-mediated somatostatin analogues for 48h were 23.1% and 22.6%, respectively. Transwell assay showed that the number of migrant A549 and SPC-A1 cells after treatment was decreased to one third. ConclusionNuclide-mediated somatostatin analogue induces apoptosis, inhibits proliferation and invasion of NSCLC A549 and SPC-A1 cells.