PLGA新型纳米支架的制备及其生物相容性

目的探讨和比较应用不同参数静电纺丝技术制备复合型聚乳酸-羟基乙酸poly(lactic-co-glycolicacid),PLGA]纳米纤维缓释膜片的可行性,检测其表面特性以及生物相容性。方法使用不同参数制备纳米纤维膜,扫描电镜观察膜片表面形态;人牙周膜细胞(human periodontal ligament cells,hPLDCs)培养及鉴定后接种于空白膜上,扫描电镜观察细胞与膜的附着情况,以MTT3-(4,5-dimethylthiaozol-2-yl)-2,5-dip henyltetrazolium bromide]实验检测不同浓度材料浸提液对细胞增殖变化情况的影响。结果随着PLGA流量增加,纳米纤维直径增加;细胞与PLGA膜复合后粘附良好且增殖状态无明显变化;不同浓度材料浸提液对牙周膜细胞增殖的影响无明显差异。结论通过不同参数静电纺丝制备的新型纳米纤维膜具有良好的生物相容性,可进一步作为多种药物及细胞因子载体构建缓释型支架,从而用于引导性牙周组织再生(guided tissue regeneration,GTR)或者牙周组织工程。

Preparation of a novel PLGA nano-fiber scaffold and its biocompatibility

Objective To prepare a novel poly(lactic-co-glycolic acid)(PLGA) electrospinning nano-fiber membrane and to test its biocompatibihty in vitro.Methods Blank nano-fiber membrane was prepared.The morphology and characteristics of membrane surface were observed with electron microscopy.Human periodontal ligament cells(HPDLCs) were cultured and identified,then inoculated onto the blank membrane.Proliferation of HPDLCs were determined by MTT method and cell adhesion to the membrane was observed by electron microscopy.Results The diameter of nanofiber was increased with the increasing flow of PLGA.hPDLCs adhered well to PLGA,and the membrane had no effect on cell proliferation.Conclusion The novel PLGA nano-fiber membrane prepared with electrospinning has good biocompatibihty in vitro,which may be used as a drug of cytokine carrier for guided tissue regeneration or periodontal tissue engineering.