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| 超声联合SonoVue介导荧光蛋白质粒转染人胚肾293T细胞的研究 | |
| 引用本文： | 郑颖,王文平,赵平,王宏卫,赵奕文.超声联合SonoVue介导荧光蛋白质粒转染人胚肾293T细胞的研究[J].同济大学学报(医学版),2008,29(6). |
| 作者姓名： | 郑颖 王文平 赵平 王宏卫 赵奕文 |
| 作者单位： | 1. 复旦大学附属中山医院超声科,上海,200032 2. 第二军医大学微生物教研室,上海,200433 3. 上海市徐汇区中心医院超声室,上海,200030 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的 探讨超声协同微泡造影剂SonoVue介导荧光蛋白质粒转染人胚肾293T细胞的方法.方法 以不同的超声辐照时间、超声机械指数和不同浓度的微泡造影剂SonoVue的参数组合,将含有绿色荧光蛋白报告基因的真核表达质粒pEGFP-N1转染人胚肾293T细胞,接种于6孔板中,以荧光显微镜观察观察293T细胞中的绿色荧光蛋白表达情况并用流式细胞仪测算转染率.结果 当微泡造影剂SonoVue浓度为40%、超声机械指数为1.0、辐照时间5 min时,质粒的转染率最高(28.11±1.63)%.结论 超声联合微泡造影刺SonoVue能够作为载体介孚质粒转染细胞,但需优化各项超声辐照的条件以达到最佳效果. |
| 关 键 词： | 超声 微泡造影剂 人胚肾细胞293T 基因转染 |
Gene transfer with pEGFP into human embryo kidney cell 293T mediated by ultrasound and SonoVue |
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| ZHENG Ying,WANG Wen-ping,HUANG Bei-jian,ZHAO Ping,ZHAO Yi-wen.Gene transfer with pEGFP into human embryo kidney cell 293T mediated by ultrasound and SonoVue[J].Journal of Tongji University(Medical Science),2008,29(6). | |
| Authors: | ZHENG Ying WANG Wen-ping HUANG Bei-jian ZHAO Ping ZHAO Yi-wen |
| Institution: | ZHENG Ying~1,WANG Wen-ping~1,HUANG Bei-jian~1,ZHAO Ping~2,ZHAO Yi-wen~3 (1.Dept.of Ultrasound,Zhongshan Hospital,Fudan University,Shanghai 200032,China,2.Dept.of Microbiology,Second Military Medical University,Shanghai 200433,3.Dept.of Ultrasound,Xuhui District Central Hospital,Shanghai 200030,China) |
| Abstract: | Objective To explore a method to transfer plasmid of enhanced green fluorescent protein (pEGFP) into human embryo kidney 293T cell mediated by ultrasound and SonoVue.Methods Under different parameters of ultrasound such as ultrasound exposure time and mechanical index(MI) and different SonoVue dosages,the expression of pEGFP was studied by fluorescence microscope and the transfection efficiency were calculated by flow cytometry.Results When the ultrasound exposure at 5 minutes and MI at 1.0 with 40%SonoVue ... |
| Keywords: | ultrasound microbubble constrast medium human embryo kidiney cell 293T gene transfection |
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