DCIK细胞特异性抗肿瘤生物学活性研究

目的研究细胞因子诱导的杀伤细胞(CIK)分别与两种冻融抗原致敏DC细胞共培养后获得的DC细胞诱导的特异性肿瘤杀伤细胞(dendritic cell induced killer.DCIK)的增殖活性及其对K562、Namalwa细胞株的杀伤活性。方法采集健康供者的外周血单个核细胞(PMNC),先诱导培养CIK细胞及两组DC细胞(K562-DC和Namalwa-DC),然后将两组DC细胞分别和CIK细胞按1∶10的比例分别混合培养,并以CIK细胞单独培养组为对照。计数细胞扩增倍数,并于共培养6d MTT法测定各组细胞对K562、Namalwa细胞杀伤活性。结果两组DCIK细胞增殖速度明显快于单纯CIK细胞组(P0.05);共培养6d,相同的效靶比情况下,两组DCIK细胞对两种肿瘤细胞的杀伤活性都比单纯CIK细胞明显增强,而用相应肿瘤抗原致敏得到的DCIK细胞对该种肿瘤细胞的杀伤活性最强。结论 DCIK细胞的增殖能力、抗肿瘤活性均高于CIK细胞,致敏的DCIK细胞对相同肿瘤靶细胞有杀伤特异性。

Specific Anti-tumor Biological Activity of DCIK Cells

Objective To investigate the proliferation activity and anti-tumor activity of two kinds of dendritic cell induced killer （DCIK） cells specifically against K562 and Namalwa cells. Methods Two groups of dendritic cells （DC）, namely DC pulsed with K562 lysate antigen （K562-DC） and DC pulsed with Namalwa lysate antigen （Namalwa-DC）, and CIK cells were induced from healthy human peripheral blood mononuclear cells. The CIK cells were co-cultured respectively with K562-DC and Namalwa-DC at a ratio of 10 to 1; CIK cells were cultured alone as controls. The cell proliferated times were counted, and killing activities against K562 and Namalwa cells were detected by MTT assay. Results The proliferation activity of two kinds of DCIK ceils was significantly higher than that of CIK cells （P〈0.05）. On the 6th day of co-cultivation in the same condition, the anti-tumor effect of DCIK cells was much higher than that of CIK cells （P〈0.05）, and tumor antigen fed DCIK cells showed the highest killing activity against the corresponding tumor cells. Conclusion The proliferation capabilities and an- ti-tumor activities of DCIK cells were higher than those of CIK cells, and DCIK cells fed by target tumor antigen have significantly enhanced specific cytotoxicity to target tumor cells.