| JNK磷酸化Bad在脑缺血神经元凋亡中的作用 |
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| 引用本文： | 王晓天,刘晓梅,尤红娟,李小翠,秦苏萍,汤仁仙,宋远见.JNK磷酸化Bad在脑缺血神经元凋亡中的作用[J].中国现代医生,2014(31):1-3. |
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| 作者姓名： | 王晓天 刘晓梅 尤红娟 李小翠 秦苏萍 汤仁仙 宋远见 |
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| 作者单位： | 徐州医学院病原生物学与免疫学教研室,江苏徐州221002 |
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| 基金项目： | 国家自然科学基金资助项目（81371300） |
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| 摘 要： | 目的探讨JNK磷酸化Bad在大鼠缺血性神经元凋亡中的作用。方法 20只大鼠均分为4组：假手术组、缺血复灌组、溶剂对照组和SP600125组。制作大鼠全脑缺血模型,应用免疫沉淀和免疫印迹法检测大鼠脑缺血复灌1 d海马CA1区神经元Bad与14-3-3及Bad与Bcl-Xl的结合、Bad丝氨酸128位磷酸化p-Bad（S128）]水平、Caspase-3蛋白表达情况。结果与缺血复灌组相比,SP600125组海马CA1区神经元Bad与14-3-3的结合明显增高,Bad与Bcl-Xl的结合、p-Bad（S128）、Caspase-3表达显著减少（均P〈0.05）。结论 JNK介导的Bad（S128）磷酸化在大鼠缺血性脑损伤中发挥了重要作用。
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| 关 键 词： | Bad JNK丝裂原活化蛋白激酶类 凋亡 脑缺血 |
Effects of Bad phosphorylation induced by JNK on cerebral ischemic neuronal apoptosis |
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| Authors: | WANG Xiaotian LIU Xiaomei YOU Hongjuan LI Xiaocui QIN Suping TANG Renxian SONG Yuanjian |
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| Institution: | (Department of Pathogenic Biology and Immunology, Xuzhou Medical College, Xuzhou 221002, China) |
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| Abstract: | Objective Investigate the effects of Bad phosphorylation induced by JNK on cerebral ischemic neuronal apoptosis in rats. Methods Twenty rats were divided into 4 groups averagely： sham operation group, ischemia-reperfusion group, sol- vent control group and SP600125 group. Making rat model of cerebral ischemia, the binding of Bad and 14-3-3 ,the binding of Bad and Bcl-X1, Bad phosphorylation at serine 128（p-Bad（S128）） and Caspase-3 protein expression in hippocampal CA1 region were detected by immunoprecipitation （IP） and immunoblotting （IB） ld after isehemia-reperfusion. Results Compared with the isehemia-reperfusion group, the binding of Bad and 14-3-3 was increased, the binding of Bad and Bcl-X1, p-Bad （S128） and Caspase-3 protein expression in hippocampal CA1 region were decreased in SP600125 group （All P〈0.05）. Conclu- sion Bad phosphorylation at serine 128 induced by JNK playe important effects on cerebral ischemic neuronal apoptosis in rats. |
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| Keywords: | Bad JNK mitogen-activated protein kinases Apoptosis Cerebral ischemia |
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