HSP27表达与胃腺癌预后相关性研究

目的检测HSP27抗体在胃腺癌中的表达，探讨HSP27的表达率与患者预后的关系。方法采集116例归档确诊为胃腺癌的石蜡包埋标本，运用免疫组织化学染色方法染色HSP27单克隆抗体，观察HSP27的表达情况。采用X。和Spearman等级相关检验的统计方法研究胃腺癌HSP27表达率与临床病理特征的关系，生存曲线采用Kaplan—Meier生存分析方法，通过多因素COX比例风险模型分析各变量对生存期的影响。结果研究发现在肿瘤病变部位及疾病的进展过程中HSP27表达失调。通过免疫组织化学染色116例胃腺癌患者组织标本，证实78例有HSP27表达，表达率为67．2％。单因素分析显示HSP27的表达率与肿瘤的TNM分期、分化程度相关（P〈0．05），研究表明HSP27低表达的胃腺癌患者生存期长于高表达的患者。多因素分析提示HSP27可作为判断胃腺癌预后的预测指标。结论本研究显示HSP27在胃腺癌组织表达升高，与肿瘤的TNM分期和分化程度呈负相关，提示HSP27表达水平可作为判断胃腺癌预后的预测指标。

Clinical Significance of HSP27 Expression in Stomach Adenocarcinoma

Objective Detection HSP27 expression in stomach adenocarcinoma for investigating HSP27 and its correlation with clinicopathological parameters in stomach adenocarcinoma. Methods Hsp27 monoelonal antibody was detected by immunohistochemical SP method in 116 cases of stomach adenocarcinoma. Statistical methods concluded χ2,Spearman,Kaplan-Meier and COX survival analysis. Results Immunohistochemical analysis showed that detectable HSP27 expression was found in 78 of 116 stomach adenocarcinoma cases （67.2%）. There was a significant correlation between HSP27 expression and TNM stage （P〈0.05）. Patients with low HSP27 expression had better survival than those with high HSP27 expression. Moreover,multivariate analysis indicated that HSP27 expression was a prognostic marker for stomach adenocarcinoma. Conclusion These results suggest that elevated expression of HSP27 protein is a frequent event during the progression of stomach aden HSP27 might be used as a potential prognostic marker for patients with stomach adenocarcinoma.