| 抗原肽HPV18 E77-15诱导产生特异性细胞毒T细胞的实验研究 |
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| 引用本文： | 张乾,徐云升,薛季森,郑飞云.抗原肽HPV18 E77-15诱导产生特异性细胞毒T细胞的实验研究[J].中国现代医生,2011,49(30):17-19. |
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| 作者姓名： | 张乾 徐云升 薛季森 郑飞云 |
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| 作者单位： | 温州医学院附属第一医院,浙江温州,325000 |
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| 基金项目： | 国家自然科学基金(编号:30500537) |
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| 摘 要： | 目的探讨HLA—A2限制性表位HPV18E77—15的免疫原性。方法对抗原肽HPV18E7-15进行T2结合分析；通过体外细胞培养技术抗原肽诱导特异性CTL扩增；采用RPE标记的表位特异性五聚体和FITC标记的CD8＋抗体通过流式细胞仪检测抗原肽所诱导产生的特异性CTLs。结果抗原肽HPVl8E77—15可将T2细胞表面HLA—A＊0201分子的表达提高2．6倍；在流式细胞仪检测中，抗原肽HPV18E77—15刺激诱导产生CD8＋五聚体＋双阳性的抗原肽特异性CTLs为1．87％。结论抗原肽HPV18E77—15能够诱导HLA—A2阳性正常人外周血淋巴细胞产生特异性CTLs，表明HPV18E77-15具有一定的免疫原性。
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| 关 键 词： | HPV18 细胞毒性T淋巴细胞 抗原肽 五聚体 |
Experimental Study on Proliferation of Specific Cytotoxic T Lymphocytes Induced by the Epitope HPV18 E77-15 |
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| Authors: | ZHANG Qian XU Yunsheng XUE Jisen ZHENG Feiyun |
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| Institution: | ZHANG Qian XU Yunsheng XUE Jisen ZHENG Feiyun The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China |
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| Abstract: | Objective To investigate the immunogenicity of the epitope HPV18 E77-15 restricted by HLA-A2.Methods T2 binding assay the epitope HPV18 E77-15 was performed;In vitro cell test,normal HLA-A2 peripheral blood lymphocytes(PBLs) from healthy HLA-A2 donor were stimulated with the epitope HPV18 E77-15 loaded autologous PBMC and T2 cells;The frequency of the epitope-specific CTLs in cultured cells were detected with the FITC-CD8+ and RPE-HLA-A2/HPV18 E77-15 pentamer in flow cytometric analysis.Results The epitope ... |
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| Keywords: | Human papillomavirus 18 Cytolytic T lymphocyte Epitope Pentamer |
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