慢性自发性荨麻疹患者外周血IL-18、IL-35的表达与意义

目的 探讨慢性自发性荨麻疹(CSU)患者外周血调节性T细胞(Treg)及辅助性T细胞1(Th1)相关细胞因子白细胞介素35(IL-35)及白细胞介素18(IL-18)的水平变化。方法 选取2019年6月至2020年10月就诊于本院皮肤科的慢性自发性荨麻疹患者50例和同时期体检中心健康者30名,采用酶联免疫吸附法(ELISA)检测外周血IL-18和IL-35表达水平,分析其水平差异及与临床症状、体征积分(UAS)之间的相关性。结果 CSU患者外周血IL-18表达水平为(125.75±19.10)pg/mL,较健康对照组的(68.96±10.65)pg/mL显著升高,与临床症状、体征积分呈正相关,差异有统计学意义(P0.05);CSU患者外周血IL-35表达水平为(41.86±6.13)pg/mL,较健康对照组的(77.38±13.19)pg/mL显著降低,与临床症状、体征积分呈负相关,差异有统计学意义(P0.05)。结论 IL-35及IL-18在CSU发病中可能发挥一定作用,可能为CSU的临床评价和治疗带来新思路。

Expression and significance of IL-18 and IL-35 in peripheral blood of patients with chronic spontaneous urticaria

Objective To explore the changes of the peripheral blood regulatory T cells (Treg) and helper T cell 1 (Th1)related cytokine interleukin-35 (IL-35) and interleukin-18 (IL-18) in patients with chronic spontaneous urticaria (CSU).Methods Fifty patients with chronic spontaneous urticaria in the dermatology department of our hospital and 30 healthy patients in the physical examination center from June 2019 to October 2020 were selected.Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of IL-18 and IL-35 in peripheral blood.The differences in IL-18 and IL-35 levels and their correlation with clinical symptoms and physical signs (UAS) were analyzed.Results The expression level of IL-18 in peripheral blood of CSU patients was (125.75±19.10)pg/mL,which was significantly higher than (68.96±10.65)pg/mL of the healthy control group,which was positively correlated with clinical symptoms and signs,and the difference was statistically significant (P<0.05).The expression level of IL-35 in peripheral blood of CSU patients was (41.86±6.13)pg/mL,significantly lower than (77.38±13.19) pg/mL of the healthy control group,which was negatively correlated with clinical symptoms and physical signs,and the difference was statistically significant (P<0.05).Conclusion IL-35 and IL-18 may play a role in the pathogenesis of CSU,which may bring new ideas for the clinical evaluation and treatment of CSU