基质金属蛋白酶-7及其组织抑制物-1与胎膜早破的相关性研究

目的 研究基质金属蛋白酶-7及其组织抑制物与胎膜早破的关系.方法 将40例孕产妇随机分为胎膜早破组20例（其中未足月PROM产妇10例,足月产PROM10例）与对照组20例（为足月临床胎膜完整产妇）,取上述孕产妇胎膜组织,采用免疫组化的方法检测胎膜中基质金属蛋白酶-7及其组织抑制物-1的浓度.结果 胎膜早破组胎膜中基质金属蛋白酶-7的表达明显高于对照组（P＜0.01）,其组织抑制物-1的表达明显低于对照组（P＜0.05）,基质金属蛋白酶-7与其组织抑制物呈负相关（P＜0.05）.结论 基质金属蛋白酶-7及其组织抑制物可能与胎膜早破有关.

Evaluation of the expression and enzyme activity of matrix metallopro-teinase-7 and the tissue inhibitor of metalloproteinase -1 in fetal mem-branes during premature upture of membranes at term in humans

Objective To investigate the relationship between matrix metalloproteinase-7 （MMP-7） and the tissue in- hibitor of metalloproteinase-1 （TIMP-1 ） during premature rupture of membranes at term in humans （PROM）. Methods Subjects were devided into two groups： 20 women with premature rupture ＂of membranes （Term PROM n=10 and Preterm PROM n=10）, 20 non-PROM pregnancies were recruited as control group.The expression of MMP-7 and TIMP were immunohistochemically demonstrated in fetal membranes. Results in PROM group MMP-7 expression in mem- brane were apparently higher than those of the control group（P〈0.01 ）, In PROM group TIMP-1 expression in mem- brane were apparently lower than those of the control group （P〈0.05）,There was a negative correlation between MMP- 7 and TIMP-1 （P〈0.05）. Conclusion The occurrence of PROM is closely related to the imbalance between MMP-7 and TIMP-1.