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| 2-甲氧基雌二醇对全脑缺血大鼠HIF-1α及RTP801的作用研究 | |
| 引用本文： | 彭静华,张鸿日,黄丽娜,李云.2-甲氧基雌二醇对全脑缺血大鼠HIF-1α及RTP801的作用研究[J].中国现代医生,2012,50(1):6-8. |
| 作者姓名： | 彭静华 张鸿日 黄丽娜 李云 |
| 作者单位： | 1. 河南科技大学第一附属医院神经内科,河南洛阳,471003 2. 河南科技大学第一附属医院神经外科,河南洛阳,471003 3. 山东大学附属济南市中心医院急诊科,山东济南,250013 |
| 基金项目： | 山东省济南市科学技术发展计划项目(051082) |
| 摘 要： | 目的探讨2-甲氧基雌二醇（2ME2）对全脑缺血大鼠HIF-1α及凋亡相关基冈RTP801的作用。方法将成年雄性SD大鼠104只随机分为对照组（n=8）、全脑缺血组（n=48）和全脑缺血2-甲氧基雌二醇干预组（n=48），后两组按再灌注时间不同各分为6个亚组。采用改良的Pulsineli4-VO方法行全脑缺血大鼠模型制作。Nissl染色进行海马神经元计数，免疫组化及RT—PCR技术进行HIF-1α及RTP801mRNA表达检测。结果全脑缺血2ME2干预组与全脑缺血组相应时间点亚组相比，神经细胞计数明显增加，HIF-1α表达减少，RTP801mRNA表达减低（P〈O．05）。结论在全脑缺血急性期．2ME2抑制HIF-1α及凋亡相关基因RTP801表达，具有一定的神经保护作用。 |
| 关 键 词： | RTP801 全脑缺血 缺氧诱导因子-1，2-甲氧基雌二醇 |
Effects of 2ME2 on hypoxia-inducible factor 1α and apoptosis-related gene RTP801 in hippocampus following the global cerebral ischemia in rats |
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| Authors: | PENG Jinghua ZHANG Hongri HUANG Lina LI Yun |
| Institution: | 1.Department of Neurology,the First Affiliated Hospital to Henan University of Science and Technology,Luoyang 471003,China;2.Department of Neurosurgery,the First Affiliated Hospital to Henan University of Science and Technology,Luoyang 471003,China;3.Department of Emergency,Jinan Central Hospital Affiliated to Shandong University,Jinan 250013,China |
| Abstract: | Objective To investigate the effect of 2ME2 on the hypoxia-inducible factor 1α and apoptosis-related gene RTP801 in hippocampus following the global cerebral ischemia in rats.Methods One hundred and four adult male Sprague-Dawley rats were randomly divided into 3 groups: sham group(n=8),global cerebral ischemia group(n=48) and global cerebral ischemia + 2ME2 treatment group(n=48),and the rats of GI group and GI + 2ME2 group were sacrificed after perfusion.The 4 vessel occlusion(4-VO)global ischemia rat model was produced.Nissl staining was used to perform quantitative analysis of cell numbers.Immunohistochemistry staining and RT-PCR provided the information of the distribution of positive cells to HIF-1α protein and the expression of RTP801 mRNA.Results The number of cells from GI + 2ME2 group was significantly higher than that from GI group(P<0.05).2ME2 significantly suppressed the levels of HIF-1α(P<0.05).The expression of apoptosis-related gene RTP801 decreased significantly(P<0.05).Conclusion 2ME2 has brain protective effect on global cerebral ischemia in rats.2ME2 inhibits the elevation of hypoxia-inducible factor 1α,which inhibits the elevation of apoptosis-related gene RTP801. |
| Keywords: | RTP801 Global cerebral ischemia Hypoxia-inducible factor 1 2-methoxyestradiol |
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