| 甲基硝基亚硝基胍诱导的一种全核γH2AX染色的特性研究 |
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| 引用本文： | 余艳柯,董正伟,石为,刘广义,杨军.甲基硝基亚硝基胍诱导的一种全核γH2AX染色的特性研究[J].浙江大学学报(医学版),2006,35(6):635-641. |
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| 作者姓名： | 余艳柯 董正伟 石为 刘广义 杨军 |
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| 作者单位： | 1. 浙江大学医学院公共卫生系,浙江,杭州,310058
2. 浙江大学医学院病理与病理生理教研室,浙江,杭州,310058 |
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| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;教育部霍英东教育基金会高等院校青年教师基金;教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 目的：明确甲基硝基亚硝基胍（MNNG）诱导的全核γH2AX所代表的DNA损伤的性质。方法：利用免疫荧光方法观察人羊膜FL细胞中MNNG诱导的γH2AX焦点的形成，中性彗星实验检测DNA双链断裂的形成，碱性彗星实验检测DNA单链、双链及其他类型损伤。结果：MNNG在10mg／L时能诱导一种特殊的全核染色，1mg／L时能在一部分细胞中诱导这种全核染色，但作用比较微弱。在这类细胞中中性彗星实验不能检测到明显的DNA双链断裂的形成，而碱性彗星实验能显示有DNA损伤的存在。结论：尽管γH2AX焦点被认为是DNA双链断裂的特异性标志，这种全核染色可能代表着另外类型的DNA损伤。
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| 关 键 词： | DNA损伤 甲硝基亚硝胍 诱变剂 羊膜/细胞学 双链断裂 |
| 文章编号： | 1008-9292（2006）06-0635-07 |
| 收稿时间： | 2005-12-28 |
| 修稿时间： | 2006-05-23 |
A distinct whole nucleus stain pattern of γH2AX induced by N-methyl-N'-nitro-N-nitrosoguanidine |
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| YU Yan-ke, DONG Zheng-wei, SHI Wei.A distinct whole nucleus stain pattern of γH2AX induced by N-methyl-N''''-nitro-N-nitrosoguanidine[J].Journal of Zhejiang University(Medical Sciences),2006,35(6):635-641. |
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| Authors: | YU Yan-ke DONG Zheng-wei SHI Wei |
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| Institution: | Department of Public Health, Center for Environmental Genomics, College of Medicine, Zhejiang University, Hangzhou 310058, China. |
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| Abstract: | OBJECTIVE: To characterize the DNA damage property represented by the distinct whole nucleus stain pattern of gammaH2AX induced by N-methyl-No-nitro-N-nitrosoguanidine (MNNG). METHODS: MNNG-induced gammaH2AX foci formation in human amnion FL cells was observed by immunofluorescent microscopy. DNA double-stranded breaks (DSBs) were detected by neutral comet assay. General DNA damages were detected by alkaline comet assay. RESULT: A distinct whole nucleus stain pattern of gammaH2AX was induced by high concentration MNNG (10 mg/L). 1 mg/L MNNG also induced this type of stain pattern in a small fraction of cells, although the effect was transient. Neutral comet assay did not detect any significant DSBs formation in this type of cells, while alkaline comet assay revealed the presence of DNA damage. CONCLUSION: Although normal gammaH2AX foci were regarded as a biomarker for DSBs, the whole nucleus stain pattern might represent DNA damage other than DSBs. |
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| Keywords: | H2AX |
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