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| 钙敏感性受体活化与心肌细胞缺血/再灌注损伤凋亡的关系 | |
| 引用本文： | 严凌,朱铁兵,孙婷婷,潘世阳,王连生,陶正贤,杨志健,曹克将.钙敏感性受体活化与心肌细胞缺血/再灌注损伤凋亡的关系[J].浙江大学学报(医学版),2011,40(2). |
| 作者姓名： | 严凌 朱铁兵 孙婷婷 潘世阳 王连生 陶正贤 杨志健 曹克将 |
| 作者单位： | 1. 南京医科大学第一附属医院心脏科,江苏南京,210029 2. 南京医科大学第一附属医院临床中心实验室,江苏南京,210029 3. 南京医科大学生化实验室,江苏南京,210029 |
| 基金项目： | 江苏省高校自然科学基金资助项目(06kjb320006) |
| 摘 要： | 目的:探讨活化的钙敏感性受体与心肌细胞缺血/再灌注损伤凋亡是否有关。方法:新生鼠心肌细胞在模拟心肌缺血状态下,培养2 h后,在标准培养液中再培养24 h,从而建立一个模拟心肌缺血/再灌注模型。使用末端脱氧核苷酰基转移酶介导性dUTP切口末端标记(TUNEL),检测心肌细胞凋亡。钙敏感性受体mRNA表达通过逆转录聚合酶链式反应(RT-PCR)测定。采用免疫蛋白印迹法(Western blotting),测定Caspase-3和Bcl-2。结果:模拟I/R增强了钙敏感性受体表达及心肌细胞凋亡。GdCl3,一种特殊的CaSR激活剂进一步增强了钙敏感性受体表达及心肌细胞凋亡,而对Caspase-3和Bcl-2则分别起着正向及负向调节作用。结论:CaSR与新生鼠心肌细胞缺血/再灌注损伤及心肌细胞凋亡有关,其促进了Caspase-3而抑制了Bcl-2的表达。 |
| 关 键 词： | 心肌再灌注损伤/病理生理学 钙敏感性受体 凋亡 心肌细胞 缺血/再灌注 |
Activation of calcium-sensing receptors is associated with apoptosis in cardiomyocytes under simulated ischemia/reperfusion |
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| YAN Ling,ZHU Tie-bing,SUN Ting-ting,PAN Shi-yang,WANG Lian-sheng,TAO Zheng-xian,YANG Zhi-jian,CAO Ke-jiang.Activation of calcium-sensing receptors is associated with apoptosis in cardiomyocytes under simulated ischemia/reperfusion[J].Journal of Zhejiang University(Medical Sciences),2011,40(2). | |
| Authors: | YAN Ling ZHU Tie-bing SUN Ting-ting PAN Shi-yang WANG Lian-sheng TAO Zheng-xian YANG Zhi-jian CAO Ke-jiang |
| Institution: | YAN Ling1,ZHU Tie-bing1,SUN Ting-ting1,PAN Shi-yang2,WANG Lian-sheng1,TAO Zheng-xian3,YANG Zhi-jian1,CAO Ke-jiang1(1.Department of Cardiology,2.Department of Clinical key Laboratory,The First Affiliated Hospital of Nanjing Medical University,3.Department of Biochemistry,Nanjing Medical University,Nanjing 210029,China) |
| Abstract: | Objective:To examine the association of activation of calcium-sensing receptors(CaSR) with apoptosis in cardiomyocytes under simulated ischemia/reperfusion. Methods:Ventricular cardiomyocytes of neonatal rats were incubated in ischemia-mimetic solution for 2 h,then re-incubated in normal culture medium for 24 h to establish a model of simulated ischemia/reperfusion(I/R).Cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL assay).The expression of CaSR mR... |
| Keywords: | Myocardial reperfusion injury/physiopathol Calcium sensing receptor Atoptosis Cardiomyocyte Ishemia/reperfusion |
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