| 16 SrRNA基因聚合酶链反应检测细菌感染的研究 |
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| 引用本文： | 滕懿群,孙眉月,尚世强,洪文澜,朱方远,于钟声.16 SrRNA基因聚合酶链反应检测细菌感染的研究[J].浙江大学学报(医学版),1999(5). |
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| 作者姓名： | 滕懿群 孙眉月 尚世强 洪文澜 朱方远 于钟声 |
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| 基金项目： | 浙江省自然科学基金，卫生厅资助 |
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| 摘 要： | 目的：探索快速可靠的检测细菌感染的新方法。方法：通过自行设计合成的细菌１６ＳｒＲＮＡ基因高度保守区引物，对２０ 种标准菌株、１２ 种２７ 株临床分离的细菌株、人基因组ＤＮＡ及巨细胞病毒进行聚合酶链反应（ＰＣＲ）扩增。结果：对所测细菌株均获得３７１ ｂｐ 扩增产物，而与人基因组ＤＮＡ、巨细胞病毒无交叉阳性反应，ＰＣＲ最低能检测ｌｐｇ 大肠杆菌ＤＮＡ。结论：建立了用共同引物ＰＣＲ扩增以判断是否存在细菌感染的方法，该方法检测快速，敏感性和特异性高。
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| 关 键 词： | 16SrRNA基因 细菌 基因 聚合酶链反应 细菌感染/诊断 |
Polymerase Chain Reaction Amplification of 16 SrRNA Gene for the Detection of Bacterial Infection |
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| TENG Yi qun,SUN Mei yue,SHANG Shi qiang,et al.Polymerase Chain Reaction Amplification of 16 SrRNA Gene for the Detection of Bacterial Infection[J].Journal of Zhejiang University(Medical Sciences),1999(5). |
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| Authors: | TENG Yi qun SUN Mei yue SHANG Shi qiang |
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| Abstract: | Objective:To study a rapid and reliable method for the detection of bacteria infection.Methods:Twenty standard bacterial strains,27 clinical bacterial isolates of 12 species,total human DNA,and cytomegalovirus were studied with polymerase chain reaction (PCR) amplification using a primer pair of 16 SrRNA gene.Results:The 16 SrRNA gene primers successfully amplified DNA from the standard strains and patient's isolated bacterial strains,PCR products were 371 bp in length,but human DNA and cytomegalovirus showed no amplification products.The sensitivity of detection of amplification showed that it was possible to detect reproducibly a band with template amounts of E.coli DNA as low as 1 pg.Conclusions:PCR amplification of bacterial DNA using highly conserved sequences for the detection of bacteria is feasible,and it offers advantages of speed,sensitivity and specificity. |
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| Keywords: | SrRNA gene Genes bacterial Polymerase chain reaction Bacterial infections/diag |
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