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银杏叶片剂指纹图谱分析方法的建立
引用本文:徐亚萍,姚彤炜,江君微.银杏叶片剂指纹图谱分析方法的建立[J].浙江大学学报(医学版),2004,33(1):24-28,36.
作者姓名:徐亚萍  姚彤炜  江君微
作者单位:浙江大学药学院,药物分析与药物代谢研究室,浙江,杭州,310031
基金项目:浙江省自然科学基金,浙江省科技厅资助项目,浙江省经贸委资助项目
摘    要:目的:建立银杏叶片剂的指纹图谱测定方法.方法:应用高效液相二极管阵列法(HPLC-DAD)检测银杏叶片剂中主要化学成分,以DiamonsilTM(钻石)C18(200 mm×4.6 mm,5 μm)柱为分析柱、CH3CN和0.01 mol/L KH2PO4缓冲液(pH 2)为流动相、梯度洗脱、二极管阵列检测器检测,测定3批银杏叶片剂和银杏叶对照提取物的指纹图谱;应用相似度计算软件,将对照提取物和片剂的整体化学成分信息进行分析.结果:银杏叶对照提取物中各个成分的相对峰高与不同浓度呈现良好的线性(r>0.999),3批片剂(生产日期跨度1年)之间整体化学成分无明显差异,片剂与对照提取物有较好的相似度(>0.965).结论:该方法精确、简便,可用于各种银杏制剂的质量控制.

关 键 词:银杏叶  黄酮类  色谱法  高效液相  指纹图谱
文章编号:1008-9292(2004)01-0024-05

HPLC fingerprint of the tablets of Ginkgo biloba L
XU Ya-ping,YAO Tong-wei,JIANG Jun-wei.HPLC fingerprint of the tablets of Ginkgo biloba L[J].Journal of Zhejiang University(Medical Sciences),2004,33(1):24-28,36.
Authors:XU Ya-ping  YAO Tong-wei  JIANG Jun-wei
Institution:College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031 China.
Abstract:OBJECTIVE: To establish the method for determination of the fingerprint of tablets of Ginkgo biloba L. METHODS: HPLC-DAD was used to determine the constituents in tablets. Diamonsil C18(200 mm x 4.6 mm, 5 microm) was used as analysis column and acetonitrile/KH(2)PO(4) as mobile phase with gradient elution. The column temperature was at 24 degree. The profile of chemical constituents in control sample and tablets obtained from the chromatograms were analyzed by similarity software. RESULT: The method developed for components analysis of the standard extracts was linear within certain concentration (r>0.999). There was no difference between the fingerprints of 3 batches of products. The fingerprints of tablets and the extract showed a good similarity(>0.965). CONCLUSION: This method is accurate simple and can be used for the quality control of Ginkgo biloba L. preparations.
Keywords:Ginkgo biloba L    Flavones  Chromatography  high-pressure liquid  Fingerprints
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