| 培养毛乳头细胞bFGF、ET-1、SCF的表达及其生物学特性 |
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| 引用本文： | 吕中法,伍津津,刘荣卿,钟白玉,郑敏.培养毛乳头细胞bFGF、ET-1、SCF的表达及其生物学特性[J].浙江大学学报(医学版),2004,33(4):296-299. |
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| 作者姓名： | 吕中法 伍津津 刘荣卿 钟白玉 郑敏 |
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| 作者单位： | 1. 浙江大学医学院,附属第二医院皮肤科,浙江,杭州,310009
2. 第三军医大学,附属西南医院皮肤科,重庆,400038 |
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| 基金项目： | 浙江省科技厅资助项目，浙江省卫生厅资助项目 |
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| 摘 要： | 目的:观察不同传代培养人毛乳头细胞的bFGF、ET-1、SCF表达变化情况及对其诱导毛囊再生能力的影响.方法:采用细胞培养、免疫组织化学和原位杂交技术,对不同传代培养的毛乳头细胞的bFGF、ET-1、SCF的表达变化情况进行观察,同时观察其对诱导毛囊再生能力的影响.结果:低传代培养毛乳头细胞ET-1、SCF表达较强,传代＞6代后ET-1和SCF转为阴性;并发现毛乳头细胞表达ET-1和SCF越强,其诱导毛囊再生能力也越高.结论:毛乳头细胞诱导毛囊再生的能力与其表达ET-1和SCF的强度相关.
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| 关 键 词： | 毛囊 细胞培养 毛乳头细胞 |
| 文章编号： | 1008-9292(2004)04-0290-04 |
| 修稿时间： | 2004年4月5日 |
Expressions of bFGF,ET-1 and SCF in dermal papilla cells and the relation to their biological properties |
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| LU Zhong-fa,WU Jin-jin,LIU Rong-qing,et al.Expressions of bFGF,ET-1 and SCF in dermal papilla cells and the relation to their biological properties[J].Journal of Zhejiang University(Medical Sciences),2004,33(4):296-299. |
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| Authors: | LU Zhong-fa WU Jin-jin LIU Rong-qing |
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| Institution: | Department of Dermatology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China. |
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| Abstract: | Objective: To investigate the expression of bFGF,ET-1 and SCF in different passages of cultured dermal papilla cells (DPC),and their possible effect on biological behaviour of DPC. Methods: The expression of bFGF,ET-1 and SCF in different passages of cultured DPC was detected by immunocytochemistry and in situ hybridization. Results: The expression of ET-1 and SCF in early passages of cultured DPC was stronger,but became negative in late passages (>6 passages ). The stronger the expression of ET-1 and SCF in DPC,the higher ability of DPC to induce hair follicle regeneration. Conclusions: The expression strength of ET-1 and SCF is related to the ability of DPC inducing hair follicle regeneration. |
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| Keywords: | ET-1 SCF |
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