测定金银花中6种有机酸类化合物含量的一种新方法

目的:为解决金银花药材分析中部分对照品价格高、不易获得的问题,建立了一测多评的分析方法.方法:通过测定绿原酸(CA)、咖啡酸(CfA)的含量,以及与其它有机酸的相对校正因子(RCF),计算出其它几种有机酸的含量.结果:在一定线性范围内,CA与新绿原酸(NCA)、3,4-二咖啡酸酰奎尼酸(3,4-DCA)、3,5-二咖啡酸酰奎尼酸(3,5-DCA)、4,5-二咖啡酸酰奎尼酸(4,5-DCA)的RCF分别为5.462、5.689、2.313、2.382,CfA与NCA、3,4-DCA、3,5-DCA、4,5-DCA的RCF分别为3.941、4.103、1.669、1.718.该方法在不同实验室、不同型号的高效液相色谱仪以及不同色谱柱上进行验证,重现性满意；与外标法相比,所得结果经方差分析均无显著性差异(P＞0.05),准确度较高.结论:该方法可采用较易获得的对照品测定金银花药材中多种有机酸的含量,有助于对金银花药材进行更为全面的质量控制.

A new method for simultaneous assay of six organic acids in Lonicerae Japonicae Flos

Objective: To establish a method of quantitative analysis of multi-components by single marker(QAMS) for quality control of Lonicerae Japonicae Flos. Methods: The contents of chlorogenic acid(CA) and caffeic acid(CfA) were determined,and the relative correction factors(RCF) of other organic acids were calculated,which were used for the indirect determination. Results: The RCFs for the neochlorogenic acid(NCA),3,4-O-dicaffeoylquinic acid(3,4-DCA),3,5-O-dicaffeoylquinic acid(3,5-DCA),and 4,5-O-dicaffeoylquinic acid(4,5-DCA) were 5.462,5.689,2.313,2.382(to CA) and 3.941,4.103,1.669,1.718(to CfA),respectively.The established method was validated in different laboratories with different high performance liquid chromatography(HPLC) instruments and different chromatographic columns;the result indicated that the reproducibility was satisfactory.There was no significant difference between the established QAMS method and external reference method(P>0.05). Conclusion: The established QAMS method can be used for simultaneous determination of 6 organic acids as quality control of Lonicerae Japonicae Flos with easy available standard substances.