针刺对脑缺血再灌注大鼠皮质缺血半暗带细胞超微结构的影响

目的：观察脑缺血再灌注后，针刺对脑缺血再灌注大鼠皮质缺血半暗带细胞超微结构的影响。方法采用改良 Longa 氏线栓法制作局灶性脑缺血再灌注大鼠模型，用多聚甲醛灌注，戊而醛固定，醋酸铀和枸橼酸铅双重染色后，HU-12A 型电镜下观察大鼠皮质缺血半暗带细胞超微结构的变化。结果模型组6 h 神经元轻度损伤，染色质边集凝固，血管周围水肿，水肿渗出明显，毛细血管管腔狭窄，脑组织散在水肿，脑细胞破坏；针刺组5 d 后神经元结构正常，血管通畅，毛细血管内皮细胞无损伤，脑组织无水肿、渗出、无坏死区域，新生毛细血管增多。结论针刺能够减轻脑水肿，较少神经元细胞的损伤，促进新生毛细血管的增生，从而达到保护脑组织的目的。

Acupuncture on ultrastructure of the cortical ischemia semi-dark cells of rats with cerebral ischemia and reperfusion

Objective To observe the effects of acupuncture on penumbra ultrastructure of the cortical ischemia semi-dark cells of rats with cerebral ischemia and reperfusion. Methods Focal cerebral ischemia reperfusion rat mod-el was made by modified longaˊs thread embolism method with paraformaldehyde perfusion and fixed by e and alde-hyde. After double staining with uranyl acetate and lead citrate,HU-12A electron microscope was used to observe the ultrastructural change of the cortical ischemia semi-dark cells of rats with cerebral ischemia and reperfusion. Results In the model group,the 6 h neurons were mild damaged,with the peripheral blood coagulation,blood vessels surround-ing edema with obvious exudation,capillary lumen stenosis,cerebral tissue edema,and cerebral cell destruction. How-ever,in the acupuncture group,5D postsynaptic neurons structure kept normal with blood vessels smooth,no damage in capillary endothelial cells,no edema,exudation,nor necrosis in cerebral tissue,and the in crease of newly born blood capillary. Conclusion Acupuncture can relieve cerebral edema,the damage of neuron cells,and promote the prolifer-ation of new blood capillary,so as to protect brain tissue.