|
|||||
|
|
| 运用于embB 285 codon点突变的分子探针设计及检测研究 | |
| 引用本文： | 陈庆海,匡红,府伟灵,张波,华兴,钟敏.运用于embB 285 codon点突变的分子探针设计及检测研究[J].四川医学,2008,29(12):1597-1599. |
| 作者姓名： | 陈庆海 匡红 府伟灵 张波 华兴 钟敏 |
| 作者单位： | 1. 第三军医大学西南医院检验科,重庆,400038 2. 解放军第452医院,四川成都,610021 3. 重庆市肺科医院,重庆,400067 |
| 基金项目： | 国家自然科学基金(编号:30571775) |
| 摘 要： | 目的探讨单碱基靶点分子探针设计模型，并应用设计的分子信标探针检测结核杆菌耐乙胺丁醇embB 285 codon点突变，并与测序结果比较以验证。方法运用软件Beacon designer设计embB基因包含285codon的分子信标，应用荧光分光光度计检测embB 285 codon扩增片段与探针杂交后荧光信号，同时对扩增产物测序并作比较。结果通过荧光分光光度计观测到结核标准株及耐乙胺丁醇embB 285 codon PCR产物与分子信标杂交后荧光信号差异存在统计学意义；33株耐乙胺丁醇组与10株H37RV标准株对照组荧光信号强度比较，耐乙胺丁醇组embB 285 codon突变检出率为15％，测序法突变检出率为15％。结论分子信标技术对单碱基靶点突变具有较高的检出率；应用荧光分光光度计直接检测液相杂交荧光具灵敏、简单、准确等优点。 |
| 关 键 词： | 耐乙胺丁醇embB基因 285密码子点突变 分子信标 荧光分光光度计 |
The study on design molecular probe and detecting mutation site of embB 285 codon |
|
| CHEN Qing-hai,KUANG Hong,FU Wei-lin,et al..The study on design molecular probe and detecting mutation site of embB 285 codon[J].Sichuan Medical Journal,2008,29(12):1597-1599. | |
| Authors: | CHEN Qing-hai KUANG Hong FU Wei-lin |
| Institution: | CHEN Qing-hai1,KUANG Hong2,FU Wei-lin,et al.1.Southwest Hospital,The Third Military Medical University,Chongqing 400038,2.No.452 Hospital of PLA,Chengdu,Sichuan 610021,China |
| Abstract: | Objective To study the model of design molecular probe detecting single basyl site and detecting embB 285codon of Ethambutol resistant MTB by designed molecular beacon probe,meanwhile,to prove the way by confer to the sequencing.Methods The software,Beacon designer,was used to design the molecular beacon of embB 285 codon and detecting fluorescence signal from hybridization between the amplified product and probe,and compare between the way and sequencing of the amplification products.Results The difference... |
| Keywords: | ethambutol resistant embB gene 285 codon of mutation site molecular beacon fluorescence spectrophotometer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |