| 高氧诱导A549细胞凋亡 |
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| 引用本文： | 张勇,廖志,叶飘,韩彦青,石境懿,罗红权.高氧诱导A549细胞凋亡[J].四川医学,2014(8):935-937. |
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| 作者姓名： | 张勇 廖志 叶飘 韩彦青 石境懿 罗红权 |
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| 作者单位： | 四川省妇女儿童医院儿科,四川成都610031 |
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| 基金项目： | 四川省卫生厅科研基金资助项目（编号：100411） |
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| 摘 要： | 目的探讨高浓度氧（高氧）诱导肺泡Ⅱ型上皮细胞（A549细胞）凋亡的机制。方法以A549细胞为研究对象,将细胞随机分成正常对照组和高氧干预组。正常对照组采用正常培养,高氧干预组用含95%O2和5%CO2混合气行高氧损伤,分别检测A549细胞的细胞活力、细胞凋亡率和capase-8的表达。结果高氧干预组细胞活力OD值（0.39±0.03）明显低于正常对照组（0.57±0.08）,高氧干预组细胞凋亡率和capase-8表达的IOD值（9.55±0.04）%;（1368.44±19.61）]均明显高于正常对照组（0.26±0.04）%;（934.29±37.40）],差异均有统计学意义（P〈0.01）。结论高氧可诱导A549细胞caspase-8表达增加,导致A549细胞凋亡。
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| 关 键 词： | 高氧 细胞活力 凋亡 caspase |
Apoptosis of A549 Cells Induced by Hyperoxia. |
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| Institution: | Zhang Yong , Liao Zhi , Ye Piao. (Sichuan Pronincial Hosphal for Women and Children, Chengdu,Sichuan 610031, China) |
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| Abstract: | Objective To explore the mechanism of apoptosis of A549 cells induced by hyperoxia. Methods A549 cell was used as target cell. The cultural cells in orifice were divided into control group and hyperoxia group. The cells of control group were cultivated with normal condition, and the cells of hyperoxia group were attacked by hyperoxia exposure. After 48 hours, Cell viability was determined by MTT assay, the apoptosis rate and easpase-8 expression of the cells in the two groups were detected re- spectively. Results As compared with control group, after exposed to hyperoxia for 48 hours, cell viability was decreased, the rate of apoptosis and caspase-8 expression were increased significantly. Conclusion Hyperoxia could enhance the apoptosis of A549 cells by upregulating the expression of caspase-8. |
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| Keywords: | hyperoxia cell viability apoptosis caspase |
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