| 不同产地安息香苯甲酸含量比较 |
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| 引用本文： | 黄立华,夏厚林,程军,王建,曾南,彭颖,周颖.不同产地安息香苯甲酸含量比较[J].山东中医学院学报,2011(4):358-360. |
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| 作者姓名： | 黄立华 夏厚林 程军 王建 曾南 彭颖 周颖 |
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| 作者单位： | 成都中医药大学药学院中药材标准化教育部重点实验室,四川成都611137 |
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| 基金项目： | 国家重点基础研究发展计划（“973”计划）项目（编号：2007CB512606） |
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| 摘 要： | 目的：比较不同产地安息香中苯甲酸的含量。方法：采用高效液相色谱法,色谱柱：Agilent TC-C18（250 mm×4.6 mm,5μm）;流动相：甲醇-水-磷酸（45∶55∶0.5）;流速：1.0 ml.min–1;柱温：25℃;检测波长：228 nm。结果：苯甲酸在0.216～2.16μg范围内与其色谱峰面积呈良好的线性关系,r=0.9999,平均回收率为99.18%,RSD为0.99%。国产安息香和进口安息香中苯甲酸含量差异较小。结论：该方法简便、稳定,可为国产安息香资源的开发利用提供依据。
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| 关 键 词： | 高效液相色谱法 不同产地 安息香 苯甲酸 |
Content comparison of benzoic acid in Benzoinum from different habitats |
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| Authors: | HUANG Li-hua XIA Hou-lin CHENG Jun WANG Jian ZENG Nan PENG Ying ZHOU Ying |
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| Institution: | (The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,Pharmaceutical College of Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China) |
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| Abstract: | Objective：To compare the content of benzoic acid in Benzoinum from different habitats.Methods：An HPLC method was used,with Agilent TC-C18 column（4.6 mm ×250 mm,5 μm）,methanol-water-orthophosphoric acid（45∶55∶0.5） as mobile phase at a flow rate of 1.0 ml·min-1,column temperature was at 25 ℃ and the detection wavelength was set at 228 nm.Results：It showed a linear relationship with the peak area range from 0.216 μg to 2.16 μg（r = 0.9999）.The average recovery was 99.18%,RSD was 0.99%.There was little difference in content between homebred Benzoinum and import Benzoinum.Conclusion：The method was proved to be convinient,stable.It will provide basis for further research and development of homebred Benzoinum. |
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| Keywords: | HPLC different habitats Benzoinum benzoic acid |
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