正交试验法优选川芎总生物碱的提取工艺

目的:优选川芎总生物碱的提取工艺。方法:采用回流提取,以酸性染料比色法测定的川芎总生物碱含量为指标,考察料液比、乙醇浓度、提取温度、提取时间对提取工艺的影响,通过正交试验L9(34)确定最佳提取工艺。结果:影响川芎总生物碱提取率的主次因素为:乙醇浓度料液比提取温度提取时间;川芎总生物碱的最佳提取工艺为:15倍量80%乙醇,100℃提取2.5 h。3次工艺验证试验中川芎总生物碱平均含量为2.745 mg/g(RSD=1.66%,n=3)。结论:本实验首次采用正交试验对川芎总生物碱的提取工艺进行优化,建立的提取工艺简便可行、稳定可靠。

Optimization of Extraction Technology of Total Alkaloids from Ligusticum chuanxiong Hort.by Orthogonal Test

Objective:To optimize the extraction process of total alkaloids from Ligusticum chuanxiong Hort.Methods:Reflux method was adopted to extract total alkaloids of L.chuanxiong.Taking the content of the total alkaloids measured by acid dye colorimetry as index,solid-liquid ratio,ethanol concentration,extraction temperature,and extraction time were assayed by L9 (34) orthogonal test to investigate the optimal extraction process.Results:The order of the factors influencing the extraction rates of total alkaloids was as follows:Ethanol concentration,solid-liquid ratio,extraction temperature,and extraction time,and the optimal extraction process was as follows:Adding 15 times amount of 80％ ethanol into L.chuanxiong,then extracting for 2.5 h at 100℃ under reflux.The average content of total alkaloids was 2.745 mg/g in 3 validation tests (RSD =1.66％,n =3).Conclusion:The extraction process of total alkaloids from L.chuanxiong was optimized by orthogonal design for the first time,and this optimized technology was simple,stable and reliable with high extraction rate.