MIF在新生大鼠缺氧缺血性脑损伤中的表达及定位研究*

目的：研究巨噬细胞迁移抑制因子（MIF）在新生SD大鼠缺氧缺血性脑损伤（HIBD）大脑皮层中的表达变化及与小胶质细胞的定位关系。方法：建立HIBD模型，采用Western Blot检测缺氧缺血再灌注0h、24h、48h、72h大脑皮层中MIF的表达变化，采用免疫荧光组织染色检测缺氧缺血再灌注48h大脑皮层中MIF表达及与小胶质细胞的定位。结果：在HIBD模型中，MIF表达明显升高，在缺氧缺血再灌注48h时表达丰度最高且与小胶质细胞存在共定位关系。结论：HIBD可诱导大脑皮层中小胶质细胞MIF的表达，MIF的上调可能参与了新生大鼠缺氧缺血性脑损伤的致病过程。

The study of MIF expression and celluar localization in the cerebral cortex of neonatal rats with hypoxic-ischemic brain damage

Objective:To investigate the expression changes of macrophage migration inhibitory factor(MIF) and its celluar localization with microglia in the cerebral cortex of neonatal rats with hypoxic-ischemic brain damage(HIBD). Methods: With the establishment of hypoxic-ischemic brain damage model, Western blot was used to study the expression changes of MIF in the cerebral cortex after hypoxia-ischemia reperfusion 0h, 24h, 48h and 72h. Immunohistochemistry method was used to study the celluar localization with microglia in the injured cerebral cortex on 48h. Results:In the HIBD model, The MIF expression was significantly increased and at its peak on 48h. Immunohistochemistry demonstrated that MIF was colocalized with microglia. Conclusions:HIBD could activate microglia to express MIF and MIF might be involved in the pathogenesis of HIBD.