灵芝三萜抑制氧化应激对大鼠高脂性脂肪肝的防治作用

目的：探讨灵芝三萜抑制氧化应激防治大鼠高脂性脂肪肝的作用。方法：SD大鼠随机分为正常对照组，模型组，灵芝三萜低、中、高剂量组（GT-L、GT-M、GT-H组）和辛伐他汀组。用高脂饲料喂养建立大鼠高脂性脂肪肝模型。经12周给药后，分别测定各组血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）及肝组织中MDA，SOD，GSH-Px和T-AOC的含量；免疫印迹观察NOX2蛋白表达情况；光镜观察肝组织脂肪病变程度。结果：与模型组比较GT-M组、GTs-H组血清TC、TG、LDL含量均显著降低（P〈0．05或P〈0．011，GT-H组HDL含量显著增加（P〈0．05）；GT-M组、GT-H组肝匀浆中MDA含量较模型组相比均明显降低LP〈0．05或P〈0．01），SOD，GSH-Px和总抗氧化能力水平较模型组相比均明显上升（P〈O．05或P〈0．01）。GT-H组可明显下调NOX2蛋白表达（P〈0．05）。GT-M组、GT-H组肝细胞脂肪变性程度明显减轻。结论：灵芝三萜能降低高脂性脂肪肝大鼠血脂水平，其对大鼠高脂性脂肪肝的防治作用可能与抑制氧化应激有关。

Effect of Ganoderma Triterpene on preventing and inhibiting the oxidative stress of hyperlipidemic fatty liver in rats

Objective： To research the effect of Ganoderma Triterpene on inhibiting the oxidative stress of hyperlipidemic fatty liver in rats. Methods： SD rats were randomly divided into the normal control group （NG）,the model group （MG）,the Ganoderma Triterpene groups of low,middle and high doses （GT-LG,GT-MG and GT-HG） and the Simvastatin group （SV）.The rats were fed with high fat diet to prepare the model of hyperlipidemic fatty liver in rats. After administration for 12 weeks, rats in each group were determined with the following indexes： total cholesterol （TC）, triglyceride （TG）,high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C）in serum as well as the contents of SOD,MDA,GSH-Px and T-AOC in hepatic tissues. Histopathological changes of hepatic tissues were observed under light glass. Results： Compared with the model group,both the GT-M group and the GT-H group showed obvious decreases in TC,TG and LDL-C （P〈0.05 or P〈0.01）, while the GT-H group showed distinct increase in HDL-C （P〈0.05）. Both GT-M group and the GT-H group showed clear decreases in MDA（P〈0.05 or P〈0.01） and evident increases in SOD,GSH-Px,T-AOC（P〈0.05 or P〈0.01）. It may be related to the expressions of NOX2 proteins down-regulated by GT- HG （P〈0.05）.The GT-M group and the GT-H group proved strikingly lighter in fatty degeneration of hepatic ceils. Conclusion： Ganoderma Triterpene can remarkablely reduce the blood fat level of hyperlipidemic fatty liver in rats and efficaciously inhibit oxidative stress, showing some effects on preventing and treating hyperlipidemic fatty liver in rats.