白蛋白钴结合试验测定缺血修饰白蛋白

目的:建立缺血修饰白蛋白(IMA)测定方法,并对正常值进行初步研究。方法:在HITACHI-7600全自动生化分析仪上设计合理参数,采用白蛋白钴结合(ACB)试验测定IMA,对其分析性能进行研究;用所建方法测定100名正常人血清IMA含量,初步制定正常人IMA的参考值。结果:所建立ACB法测定低、中、高浓度IMA的批内CV分别为2.52%、2.41%、1.96%,批间CV分别为2.56%、3.42%、3.06%;白蛋白浓度在20~50g/L范围内呈线性关系,方法的精密度和线性均佳。正常人IMA95%参考上限值为:73.0ABSU/ml。结论:在HITACHI-7600全自动生化分析仪成功建立ACB法测定IMA的方法。

Measurement of Ischemia Modified Albumin by Albumin Cobalt Binding Test

Objective:To establish albumin cobalt binding test for measurement of ischemia modified albumin (IMA) and study its normal reference values. Methods:IMA were detected by HITACHI-7600 autobiochemical analyzer with albumin cobalt binding test and evaluated its analytical performance. Study of IMA reference values were established with 100 healthy controls. Results:The coefficient of variation values of both intra-experimental and inter-experimental reproducibility for low concentration sample were 2.52% and 2.56%, and those for medium concentration sample were 2.41% and 3.42%,and those for high concentration sample were 1.96% and 3.06% respectively. The detection range of the assay was from concentration of albumin 20.0 g/L to 50.0g/L. 95%upper limit values were 73.2ABSU/1ml. Conclusions:This assay had high sensitivity and reproducibility, and suitable for clinical practice, which will certainly play a significant role in approaching relationship between IMA and associated diseases.