大鼠坐骨神经缺损后背根神经节组织lncRNA表达变化 |
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引用本文: | 钱天梅,高蓉,于彬,丁斐,顾晓松. 大鼠坐骨神经缺损后背根神经节组织lncRNA表达变化[J]. 中国交通医学杂志, 2012, 26(4) |
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作者姓名: | 钱天梅 高蓉 于彬 丁斐 顾晓松 |
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作者单位: | 钱天梅 (南通大学江苏省神经再生重点实验室,江苏,226001) ; 高蓉 (南通大学江苏省神经再生重点实验室,江苏,226001) ; 于彬 (南通大学江苏省神经再生重点实验室,江苏,226001) ; 丁斐 (南通大学江苏省神经再生重点实验室,江苏,226001) ; 顾晓松 (南通大学江苏省神经再生重点实验室,江苏,226001) ; |
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基金项目: | 国家863计划项目,国家自然科学基金资助项目 |
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摘 要: | 目的:研究大鼠坐骨神经缺损后背根神经节中长链非编码RNA(long noncoding RNA,lncRNA)的表达变化。方法:建立SD大鼠坐骨神经损伤0天、1天、4天和7天模型,取背根神经节组织进行lncRNA检测,进行差异基因的筛选,并应用实时荧光定量逆转录-多聚酶链反应(qRT-PCR)对芯片结果进行验证;利用lncRNA与mRNA特异性表达的标准化信号强度,构建基因共表达网络。结果:共筛选出24个显著下调的lncRNAs,qRT-PCR检测的lncRNA AF130881和AB020616的表达变化与芯片一致。得到与lncRNA AF130881和AB020616正向或负向共表达的蛋白编码基因25个。结论:大鼠坐骨神经缺损后背根神经节组织中lncRNA的表达谱发生改变。
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关 键 词: | 坐骨神经缺损 背根神经节 长链非编码RNA 实时荧光定量逆转录-多聚酶链反应 大鼠 |
Altered lncRNA expression following sciatic nerve resection in rats |
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Abstract: | Objective: To analyze the expression of lncRNA in the dorsal root ganglia(DRGs) after resection of the sciatic nerve in rats.Methods: The expression of lncRNAs was examined in a sciatic nerve resection model at 0 d、1 d、4 d and 7 d by using an rat lncRNA array.The lncRNA expression was also confirmed by quantitative real-time polymerase chain reaction(qRT-PCR).Gene coexpression network was constructed according to the normalized signal intensity of specific expression lncRNAs and mRNAs.Results: A total of 24 lncRNAs were identified which were significantly down-regulated in the DRGs after resection from 0 through 1 and 4 until 7 d.The expression of lncRNA AF130881 and AB020616 analyzed by qRT-PCR was in agreement with the microarray data.The network was constructed from lncRNA AF130881 and AB020616 positively or negatively coexpressed with 25 coding mRNAs at the serial time points.Conclusion: This study demonstrated a different involvement of lncRNAs in the DRGs after resection of the sciatic nerve in a rat model. |
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Keywords: | sciatic nerve resection dorsal root ganglia long noncoding RNA Quantitative real-time polymerase chain reaction rats |
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