胰腺癌中VEGF表达、微血管生成与生物学行为关系的研究

目的:探讨胰腺癌组织中血管内皮生长因子(VEGF)的表达、微血管密度(MVD)与肿瘤分化程度、淋巴结转移的关系。方法:应用免疫组化Envision法检测38例胰腺癌组织中VEGF表达情况,以CD34单克隆抗体标记肿瘤血管内皮细胞测定其MVD,并与临床病理特征对照分析。结果:胰腺癌组织中VEGF表达阳性率73.6%,微血管密度49.71±20.5,显著高于正常胰腺组织的VEGF表达及MVD(24.3±8.4);低分化癌和(或)伴淋巴结转移的胰腺癌VEFG阳性率(83.33%,71.43%)及MVD(69.52±14.52,69.73±16.8)显著高于中、高分化胰腺癌和无淋巴结转移的胰腺癌(37.5%;50.0%;38.89%),(34.24±16.9;41.03±19.28;34.71±15.5)。VEGF表达阳性的癌组织MVD为60.84±21.2,明显高于VEGF表达阴性的癌组织35.8+12.2,两者间差异有统计学意义(P<0.05)。结论:VEGF过度表达在胰腺癌微血管生成过程中起重要作用,VEGF的表达及MVD与胰腺癌组织学分级、生物学行为有密切关系,可作为反映胰腺癌预后的一个重要参考指标。

Expression of VEGF and the correlation between tumor angiogenesis,and lymph node metastasis in pancreatic carcinoma

Objective: To study the relationship between tumor micro-angiogenesis,expression of VEGF and lymph node metastasis,differrentiation,etc.in pancreatic carcinoma(PCA).Methods: Envision immunohistochemical and CD34 antibody was used to detect the micro-vessel density(MVD) in pancreatic carcinoma and normal pancreatic tissue and analyse MVD with clinical pathological characteristics.Results: MVD in pancreatic carcinoma was 49.71±20.5,while MVD in normal pancreas tissue was 24.3±8.4.MVD in PCA of low differentiation was higher than that in PCA of high differentiation.MVD in PCA with lymph node metastasis was higher than that in PCA without lymph node metastasis(69.52±14.52、34.24±16.9,P<0.01;69.73±16.8、34.71±15.5,P<0.01).Conclusion: MVD is higher in pancreatic carcinoma than that in normal pancreatic tissue.MVD is closely related to tissue classification and biological behavior of pancreatic carcinoma.