| 20（S）-原人参二醇对人胚肾293细胞生长的抑制作用 |
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| 引用本文： | 翟旭光,陈广通. 20（S）-原人参二醇对人胚肾293细胞生长的抑制作用[J]. 南通医学院学报, 2010, 30(5): 315-318,F0002 |
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| 作者姓名： | 翟旭光 陈广通 |
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| 作者单位： | 南通大学医学院生命化学教研室,南通,226001 |
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| 基金项目： | 南通大学引进人才科研启动经费 |
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| 摘 要： | 目的：研究20（S）-原人参二醇（PPD）对人胚肾293细胞（HEK-293细胞）生长抑制作用。方法：采用台盼蓝染色和噻唑蓝（MTT）法观察PPD对HEK-293细胞增殖的抑制作用,同时通过Hoechst33258染色和DNA Ladder方法测定PPD对HEK-293细胞凋亡影响。结果：PPD可明显降低HEK-293细胞存活率和细胞活性,半数抑制浓度（IC50）为21.8μmol/L。PPD处理后HEK-293细胞核出现典型的凋亡形态学的改变,琼脂糖电泳呈现明显的DNALadder条带。结论：PPD对HEK-293细胞生长有明显的抑制作用并有剂量依赖关系。
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| 关 键 词： | 20（S）-原人参二醇 人胚肾293细胞 Hoechst33258 噻唑蓝 |
Inhibition of 20(S)-protopanaxadiol on cell growth of human embryomic kidney 293 cells |
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| ZHAI Xuguang,CHEN Guangtong. Inhibition of 20(S)-protopanaxadiol on cell growth of human embryomic kidney 293 cells[J]. ACTA Academiae Medicinae Nantong, 2010, 30(5): 315-318,F0002 |
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| Authors: | ZHAI Xuguang CHEN Guangtong |
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| Affiliation: | (Biochengistry Laboratory,Medical College of Nantong University,Nantong 226001) |
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| Abstract: | Objective：To study the effects of 20 （S）-protopanaxadiol （PPD） on the on cell growh of HEK-293 cell line.Methods：HEK-293 cell line was treated with PPD.The activity of HEK-293 cells was determined with MTT assay.The effects of PPD on apoptosis rate were observed by the Hoechst 33258 dye and DNA ladder.Results：20 （S）-Protopanaxadiol could inhibit the proliferation of HEK-293 cell,IC50 values was 21.8 μmol/L.The morphological changes were observed in the nuclei of HEK-293 cells treated by PPD.The DNA Ladders bands were detected by 1% agrose gel electrophoresis.Conclusion：20（S）-Protopanaxadiol could inhibit the proliferation of HEK-293 cell in a dose dependent manner. |
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| Keywords: | 20 （S）-protopanaxadiol （PPD） Human embryomic kidney-293 cell（HEK-293 cell） Hoechst 33258 3-（4 5dimethylthiazol-2-yl）-2 5-diphenyltetrazolium bromide （MTT） |
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