雷公藤内酯醇抑制胰腺癌细胞增殖和诱导凋亡的研究

目的：探讨雷公藤内酯醇（TL）对胰腺癌细胞增殖和凋亡的影响及其机制。方法：胰腺癌细胞SW1990与TL共同孵育,采用MTT法观察TL对胰腺癌细胞增殖的抑制作用,以流式细胞仪AnnexinⅤ/碘化丙啶（PI）双染法检测TL对SW1990细胞的凋亡的诱导作用和对细胞周期的干预作用。结果：TL抑制SW1990细胞增殖和促进细胞凋亡,呈现为浓度和时间依赖性,TL阻滞胰腺癌细胞周期于G0/G1期。结论：TL能抑制胰腺癌细胞增殖、诱导细胞凋亡及干预胰腺癌细胞周期,TL具有潜在的肿瘤治疗作用。

The effects of triptolide on the proliferation and apoptosis of pancreatic cancer cell

Objective：To investigate the effects and the possible mechanism of triptolide on the growth and apoptosis of human pancreatic cancer cell in vitro.Methods：The pancreatic cancer cell line SW1990 was treated with triptolide.MTT method was used to determine the inhibitory effect of triptolide on cell proliferation,and flow cytometry （FCM） with AnnexinⅤ-FITC and propidium iodide （PI） staining was used to determine apoptosis induction and blocking effects of triptolide on the cycle proliferation.Results：After the treatment with triptolide,cell proliferation was blocked by triptolide,which showed a significant positive association with the level of triptolide concentration（P0.01）,and the apoptotic rates of SW1990 cells were increased in concentration-dependent and time-dependent manners,and its cell cycle was arrested at G0/G1 phase.Conclusion：Triptolide is able to inhibit proliferation,induce the apoptosis and block cell cycle effect on pancreatic cancer cells.The potential for its treatment is worth further investigating.