| dd—PCR法研究中草药促神经生长过程中的基因差异表达 |
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| 引用本文： | 刘梅,顾晓松.dd—PCR法研究中草药促神经生长过程中的基因差异表达[J].南通医学院学报,1999,19(4):363-365. |
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| 作者姓名： | 刘梅 顾晓松 |
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| 作者单位： | 南通医学院!南通226001(刘梅，刘炎，崔学芝，邱一华)，南通医学院生物学教研室!南通226001(顾晓松，张沛云) |
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| 基金项目： | 国家杰出青年科学基金!资助 ( 3 94 2 5 0 0 6)，交通部通达计划!资助 ( 95 0 5 0 4 16) |
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| 摘 要： | 目的 :为探讨中药促神经生长的分子机理提供实验依据。方法 :采用 dd-PCR方法 ,从体外培养大鼠背根神经节细胞加中药组和不加中药组中获得两者的差异表达片段 ,并克隆测序分析。结果 :获得 7个差异条带 ,其中 2个条带的 c DNA序列分别与酪氨酸磷酸酶受体 ( PTPR) m RNA、促生长素抑制素受体 ( SSTR) m RNA的序列部分同源。结论 :在中药促神经生长过程中 ,神经元基因具有选择性表达的变化
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| 关 键 词： | 中药 神经生长 dd-PCR 基因表达 |
THE PRELIMINARY STUDY OF GENE DIFFERENTIAL EXPRESSION UNDER THE EFFECT OF CHINESE HERB ON NERVE GROWTH |
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| Liu Mei,Gu Xiaosong,Liu Yan,Cui Xuezhi,Qiu Yihua,Zhang Peiyun.THE PRELIMINARY STUDY OF GENE DIFFERENTIAL EXPRESSION UNDER THE EFFECT OF CHINESE HERB ON NERVE GROWTH[J].ACTA Academiae Medicinae Nantong,1999,19(4):363-365. |
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| Authors: | Liu Mei Gu Xiaosong Liu Yan Cui Xuezhi Qiu Yihua Zhang Peiyun |
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| Abstract: | Objective: To explore the molecular mechanism of the effect of Chinese herb upon nerve growth .Methods: The differential display of cDNA fragments had been obtained from two kinds of cultured cells from dorsal root ganglia - with Chinese herb and without Chinese herb by dd-PCR. Then, cDNA fragments were cloned and sequenced automatically. Results: Seven differential fragments were observed between the two kinds of cultured cells. Sequence of these fragments had been detected. Homology analysis with GenBank BLAST 2.0 showed that one shared some homology with PDPR, and the other had some homology with SSTR.Conclusion: Under the effect of Chinese herb on nerve growth, correlative gene displays differential expression. |
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| Keywords: | Chinese herb nerve growth dd-PCR gene expression |
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