| OmpC-HBsAg‘a’表位嵌合蛋白疫苗的构建表达及其对HBV转基因小鼠的免疫效果研究 |
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| 引用本文： | 钮晓音,倪润洲,李小彦.OmpC-HBsAg‘a’表位嵌合蛋白疫苗的构建表达及其对HBV转基因小鼠的免疫效果研究[J].南通医学院学报,2011(4):243-247,F0002. |
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| 作者姓名： | 钮晓音 倪润洲 李小彦 |
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| 作者单位： | 上海交通大学医学院上海免疫学研究所;南通大学附属医院消化内科; |
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| 基金项目： | 南通市社会发展项目(S2009021); 上海交通大学医学院科技基金资助项目(09XJ21063); 上海市卫生局青年科研项目(2008Y019) |
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| 摘 要： | 目的：构建OmpC-HBsAg‘a’表位嵌合蛋白疫苗来研究对HBV转基因小鼠的免疫效果。方法：将HBsAg‘a’抗原决定簇主要肽段S-（124-147aa）插入到沙门氏菌外膜孔道蛋白OmpC第4 Loop区,并运用DsbA进行辅助二硫键形成,对HBV转基因小鼠免疫,激活固有免疫系统中的B-1细胞产生HBsAb。结果：HBsAg的adr‘a’表位CTSPAQGTSMF-PSCCCTKPSDGNC成功插入OmpC-HBsAg‘a’基因的588~659 bp之间,重组后的载体PCR鉴定和测序结果完全与设计相一致。IPTG诱导表达后,荧光显微镜显示3E7抗体能够识别BL21外膜表面的OmpC-HBsAg‘a’表位嵌合蛋白,用表达该蛋白的BL21直接免疫HBV转基因小鼠,能够产生HBsAb抗体。结论：OmpC-HBsAg‘a’表位嵌合蛋白在原核中表达具有天然构象,以此蛋白为基础的疫苗能够打破HBV转基因小鼠对HBsAg的免疫耐受。
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| 关 键 词： | 嵌合蛋白疫苗 OmpC 乙型肝炎病毒转基因小鼠 免疫 |
Construction and expression of OmpC-HBsAg 'a' epitope chimeric protein vaccine and study of its immune effect on HBV transgenic mice |
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| NIU Xiaoyin,NI Runzhou,LI Xiaoyan.Construction and expression of OmpC-HBsAg 'a' epitope chimeric protein vaccine and study of its immune effect on HBV transgenic mice[J].ACTA Academiae Medicinae Nantong,2011(4):243-247,F0002. |
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| Authors: | NIU Xiaoyin NI Runzhou LI Xiaoyan |
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| Institution: | NIU Xiaoyin1,NI Runzhou2,LI Xiaoyan2(1Shanghai Jiaotong University Medical School,Shanghai Institute of Immunology,Shanghai 200025,2Department of Gastroenterology,Affiliated Hospital of Nantong University) |
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| Abstract: | Objective: To study the immune effect of OmpC-HBsAg 'a' epitope chimeric protein vaccine on HBV transgenic mice.Methods: The major peptide of antigenic determinant from HBsAg 'a',S-(124-147aa),was inserted into the fourth Loop of OmpC,the outer membrane pore protein of Salmonella,then disulfide bond was formed by the assistant of DsbA.When HBV transgenic mice were immunized with OmpC-HBsAg 'a' epitope chimeric protein vaccine,B-1 cells were activated and secreted HBsAb in their native immune system.Results:... |
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| Keywords: | chimeric protein vaccine OmpC heptitis B virus transgenic mice immunization |
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