1996～1997年江西省南昌地区流感监测

为了监测流感动态确定优势病毒株，在江西省南昌地区进行了流感病毒分离和鉴定研究。从1996年5月～1997年4月共采取感冒病人鼻咽拭子1440份，全部用双腔接种法接种鸡胚，部分标本又进行细胞培养。采用血凝抑制试验（HAI）鉴定病毒株的型及亚型，对细胞培养获得的20株病毒同时应用间接免疫荧光试验（IFA）鉴定病毒株的型别，取HAI法检测为甲型的任意20株进行多聚酶链反应（PCR）试验。结果：共分离得流感病毒47株，其中，甲1型（H1N1）26株，占55．32％；甲3型（H3N2）10株，占21．28％；乙型（B）11株，占23．4％，优势流行株是甲1型，发现了甲1、甲3、乙型在同一地区同时流行的现象，也发现了“O”相毒株。因流感病毒的变异均局限在量变范围内，预计短期内没有大流行的危险。IFA法PCR试验结果全部与HAI法一致，故结论可靠，IFA、PCR法也是快速准确鉴定流感病毒的好方法。

Surveillance of Influenza in Nanchang Area of Jiangxi Province between 1996 and 1997

To survey flu and establishing prevailing strains, the flu viruses in Nanchang Area of Jiangxi Province were isolated and identified. From May 1996 to April 1997, 1 440 nose and throat swab saniples from patients suffering cold were collected. All these samples were inoculated to 10-day-embryonated chickens for isolating flu virused . Some of them were inoculated into MDCK cells again. All obtained viruses strains were identified by hemagglutinin inhibition assay (HAI). Of them, 20 strains were also identified by indirect immunofluorescence assay (IFA) and 20 type A strains were assayed by polymerase chain rection (PCR) technique. Of 47 flu virus strains obtained, 26 were H1N1 (55. 32%), 10 H3N2(21. 28%) and 11 type B (23. 4%). The prevailing strain was H1N1. Viruses strains of H1N1, H3N2 and type B were usually discovered in same region and season . " O " phase viruses strains were also discovered, but the genetic shift of flu viruses was undiscovered . It is indicated that the flu will not break out epidemically in the near future. The results of IFA and PCR coincide with HAI. The conclusion is that IFA and PCR are quick and accurate for identifying flu viruses.