环孢菌素A、汉防己甲素及两者联合逆转K562／A02细胞的多药耐药

目的：探讨环孢菌素A（CsA）、汉防己甲素（Tet）及两者联合应用对人白血病耐药细胞系K562／A02多药耐药的逆转作用。方法：采用M1Tr法测定柔红霉素（DNR）对细胞的半数抑制量（IC50），流式细胞仪检测细胞凋亡率和细胞内DNR浓度。结果：DNR对K562细胞和K562／A02细胞IC50分别为0．27和21．22mg·L^-1；CsA（1mg·L^-1）及Tet（0．1mg·L^-1）单独和联合处理K562／A02后，DNR的IC50分别为7．83、5．32和2．32mg·L^-1；CsA和Tet对DNR作用K562的IC50无明显影响；K562／A02细胞48h凋亡率为2．19％，DNR（1mg·L^-1）作用K562／A02细胞凋亡率为2．70％，CsA和Tet单独及联合处理后凋亡率分别为10．27％、17．64％和52．79％；两药处理后细胞内化疗药物DNR浓度亦明显增加。结论：CsA及Tet均可逆转耐药，且联合作用逆转效果更强。

In Vitro study of the effect of cyclosporine A,tetrandrine and their combination on the reversion of multidrug resistance in leukemia cell line

To study the reversible effect of cyclosporine A（ CsA）, tetrandrine（Tet） and their combination on multidrug resistance of cell line K562/A02. Methods The IC50 of DNR was determined by MTT assay. The apoptotic changes and the intracellular DNR concentration were observed by flow cytometry. Results Treating K562/A02 cells with CsA（ 1 mg·L^-1 ） ,Tet（0.1 mg·L^-1） and both for 48 hours partially restored the sensitivity of K562/A02 cells to DNR, but had no effect on K562 cells;K562/A02 cells showed apoptotic characteristics after treated with CsA and both for 48 hours; CsA and Tet （alone or combination） elevated the intracellular DNR concentration in K562/A02 cells. Conclusion Multidrug resistance can be partially reversed by CsA or Tet, the combination of both drugs shows a great reversal effect.