再生障碍性贫血患者血清抑制CFU-MK的增殖及调控

目的:探讨再生障碍性贫血(再障)患者血小板减少的原因,建立模型并寻找治疗对策。方法:应用甲基纤维素半固体体外培养法建立CFU-MK培养体系;混合再障血清进行培养建立再障患者血小板减少的模型;使用不同的造血细胞生长因子进行组合观察模型中CFU-MK集落生成情况和集落中巨核祖细胞的含量。结果:经过14d的培养,模型组CFU-MK集落、集簇数量明显低于正常对照组,以TPO/IL-3/IL-11/FL细胞因子组合使模型组CFU-MK的数量恢复及巨核细胞的扩增效果最好。结论:本实验建立了CFU-MK体外培养体系及再障血小板减少的模型,体外试验表明治疗再障血小板减少以TPO/IL-3/IL-11/FL为最佳组合。

The proliferation and regulation of CFU-MK were inhibited by sera from patients with aplastic anemia

Objects:To investigate the reasons and establish the model of thrombopenia of aplastic anemia,and explore the treatment strategy of thrombopenia of aplastic anemia.Methods:CFU-MK and the model of thrombopenia of aplastic anemia were analysed by methylcellulose culture or with sera from patients with aplastic anemia.The number of megakaryocyte colony-forming unit and the content of CD41 cells in CFU-MK were measured simultaneously.Results:After 14 days of culture,the number of CFU-MK and megakaryocyte cluster-forming unit of the model groups were significantly less than that of the normal control groups.The combination of TPO/IL-3/IL-11/FL was the most suitable for the growth of CFU-MK and the expansion of megakaryocytic progenitors incultured with sera from patientswith aplastic anemia.Conclusions:It is concluded that the study has established the model of thrombopenia of aplastic anemia and the methods of culture of CFU-MK,The combination the TPO/IL-3/IL-11/FL was the best optional for the treatment of the thrombopenia of aplastic anemia in vitro.