白藜芦醇对小鼠肺泡巨噬细胞释放IL-6、TNF-α的影响

目的:探讨白黎芦醇(Res)对小鼠肺泡巨噬细胞释放细胞因子IL-6,TNF-α的影响。方法:经从小鼠支气管肺泡灌洗中获得肺泡巨噬细胞,分5组进行培养,A组(LPS 10mg/L),B组(LPS 10mg/L+Res 50μg/ml),C组(LPS 10mg/L+Res 100μg/ml),D组(LPS 10mg/L+Res 150μg/ml),E组(LPS 10mg/L+Res 200μg/ml).各组在培养4,6,12,24h提取上清液,应用ELISA法测定上清液中IL-6,TNF-α含量。结果:在LPS刺激下IL-6于12h达释放高峰,在高峰期随着白黎芦醇浓度的逐步加大,IL-6的含量相应逐渐降低,E组IL-6含量明显低于只有内毒素刺激的A组(P<0.05)。同样在LPS刺激下TNF-α于6h达释放高峰,在高峰期随着白黎芦醇浓度的逐步加大,TNF-α含量也相应逐渐降低,而C组、D组、E组TNF-α含量明显低于A组(P<0.05)。结论:白黎芦醇对小鼠肺肺泡巨噬细胞过度释放炎症性细胞因子IL-6,TNF-α具有明显抑制作用。

Effect of resveratrol on IL-6 and TNF-α released by alveolar macrophages

Objective: To observe the effect of resveratrol on interleukin-6(IL-6) and tumor necrosis factor(TNF-α) released by alveolar macrophages.Methods : Alveolar macrophages were isolated from bronchoalveolar lavage(BAL) fluid from mice.The macrophages were cultured and stimulated with lipopolysaccharide(LPS,10μg/ ml) and different concentration of resveratrol.A groups were lipopolysaccharide 10mg/L.B group were LPS 10mg/L and Res 50μg/ml.C group were LPS 10mg/L and Res 100μg/ml.D group were LPS 10mg/L and Res 150μg/ml,E group were LPS 10mg/L and Res 200μg/ml.After 4,6,12,24 hours,the IL-6 and TNF-α were measured by ELISA in the supernatant.Results: After endotoxin stimulation,IL-6 levels peaked at 12 hours,the IL-6 level in E group was significantly lower than that of A group(P < 0.05).TNF-α levels peaked at 6 hours,the TNF-α levels in C,D and E groups were significantly lower than that of A group(P < 0.05).Conclusion: Resveratrol can inhibit the endotoxin induced inflammatory cytokine secretion in alveo larmacrophages.