新城疫病毒对体外培养人膀胱癌EJ细胞凋亡作用机制研究

目的探讨新城疫病毒(NDV)对体外肿瘤细胞的抑制作用机制。方法以人膀胱癌EJ细胞株为靶细胞,观察NDV对人膀胱癌EJ细胞的作用。结果NDV病毒能作用于体外培养膀胱癌细胞,光镜下细胞的贴壁率和生存率显著下降,电镜下可见到凋亡典型形态结构,TUNEL方法检测膀胱癌细胞有大量凋亡,新城疫病毒作用膀胱癌细胞24h凋亡细胞阳性指数(AI)为9.9%,48h凋亡细胞阳性指数(AI)为17.8%。免疫组化检测膀胱癌细胞有p53、bax、Fas、Fas-L及细胞因子TGFβ1表达增加而原癌基因bcl-2的表达降低从而诱导膀胱癌细胞凋亡。结论NDV病毒是一种有效抗肿瘤细胞的病毒,在体外具有明显的抑制肿瘤细胞作用。

Study on the suppressing effect of NDV on cultured bladder carcinoma cells (BST739) in vitro

Objective: To explore the suppressing effect of Newcastle disease virus(NDV) on human bladder carcinoma cells (BST_~739 ) in vitro.Methods:Human bladder carcinoma cells(BST_~739 ) were regarded as target cells to observe the suppressing effect of NDV on them. The cell growth and proliferation were measured by MTT assay method. Cell apoptosis was evaluated by electron microscope and in situ TUNEL(TdT-mediatde dUTP-X nick end labeling) . The expression of cell apoptosis associated genes was examined by immunohistochemical staining. Results:NDV had an effect on tumor cells and finally caused their apoptosis. The cell growth and proliferation were inhibited obviously, depending on time and concentration. The histological and ultrastructural changes were observed by electron microscope and microscope in treated group. The apoptosis index was 9.9% and 17.8% at 24 and 48h in situ TUNEL respectively in treated group. Human bladder carcinoma cell(BST_~739 ) apoptosis was induced and the protein expression of cancer gene bcl-2 was down regulated , while that of p53, bax, Fas, Fas-L genes and cell factor TGFβ_1 were up regulated after NDV culture. Conclusion:NDV might be an effective oncolytic agent to bladder carcinoma cells(BST_(739)).It has an obvious direct suppressing effect on tumor cells in vitro.